1.

Which steps are involved in genetic engineering?

Answer»

Genetic engineering process has several steps:

(i) Gene isolation: The desired is cut with restriction enzymes and joined by ligases to host DNA.

(ii) Synthesis of gene: The nucleotides are synthesized chemically.

(iii) Transformation: The DNA is incorporated into genome of a cell.

(iv) Taunsduction: The DNA is carried from a donor bacterium to  a recipient bacterium with the help of bacteriophages. It results in changing the recipient's genotype.

(v) Gene cloning: It is a technique for producing new combination of genetic material in bacterial cells. The DNA is inserted in the vector, where it produces copies of itself. 



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