Which type of DNA library would you prefer for liver cells. Give prope

1.	Which type of DNA library would you prefer for liver cells. Give proper explanation for making such a library.
Answer» c DNA library would be preferred. mRNA molecules are highly unstable as they are easily degraded by RNAses .Therefore mRNA molecules are copied into the more stable DNA (now called cDNA) before cloning. The construction of a cDNA library begins with the isolation of mRNA from a given cell type or tissue which are copied into cDNA using a special enzyme called reverse transcriptase. The procedure results in double-stranded cDNA which can be incorporated into vectors such as pBR322.These recombinant vectors are transformed into host bacterial cells eg. E.coli. This forms a cDNA library.

Which type of DNA library would you prefer for liver cells. Give proper explanation for making such a library.

Answer»

c DNA library would be preferred.

mRNA molecules are highly unstable as they are easily degraded by RNAses .Therefore mRNA molecules are copied into the more stable DNA (now called cDNA) before cloning. The construction of a cDNA library begins with the isolation of mRNA from a given cell type or tissue which are copied into cDNA using a special enzyme called reverse transcriptase. The procedure results in double-stranded cDNA which can be incorporated into vectors such as pBR322.These recombinant vectors are transformed into host bacterial cells eg. E.coli. This forms a cDNA library.

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