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Which Type Of High Performance Liquid Chromatography Technique Is Most Widely Used? |
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Answer» Reverse phase Chromatography has the widest range of applications. The stationary phase COMPRISES non polar organic chains bound to inert silica surface and MOBILE phase comprises of aqueous or aqueous-organic MIXTURES comprising of polar solvents of varying degrees of polarity. The elution sequence is polar followed by LESS polar and least polar or non polar COMPOUNDS eluting last through the column. Reverse phase Chromatography has the widest range of applications. The stationary phase comprises non polar organic chains bound to inert silica surface and mobile phase comprises of aqueous or aqueous-organic mixtures comprising of polar solvents of varying degrees of polarity. The elution sequence is polar followed by less polar and least polar or non polar compounds eluting last through the column. |
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