While creating the first Recombinant DNA why was it necessary for linking the gene of interest with the plasmid of Salmonella typhimurium when E coli already have their own plasmids??? Why can't we directly introduce our gene of interest in ecoli medium so that our gene of interest is directly in the plasmid of ecoli itself??

While creating the first Recombinant DNA why was it necessary for link

1.	While creating the first Recombinant DNA why was it necessary for linking the gene of interest with the plasmid of Salmonella typhimurium when E coli already have their own plasmids??? Why can't we directly introduce our gene of interest in ecoli medium so that our gene of interest is directly in the plasmid of ecoli itself??
Answer» While creating the first Recombinant DNA why was it necessary for linking the gene of interest with the plasmid of Salmonella typhimurium when E coli already have their own plasmids??? Why can't we directly introduce our gene of interest in ecoli medium so that our gene of interest is directly in the plasmid of ecoli itself??