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Why difference in ph of buffer in sds gel electrophoresis? |
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Answer» The stacking gel has a low concentration of acrylamide and the running gel a higher concentration capable of retarding the movement of the proteins. ... When the power is TURNED on, the negatively-charged glycine ions in the pH 8.3 electrode BUFFER are FORCED to ENTER the stacking gel, where the pH is 6.8 |
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