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Why do ddNTP’s cause chain termination during Sanger’s DNA sequencing method? Write the DNA fragments formed by chain termination for the given original DNA strand-3’ ATGCTAGC 5’. |
Answer» 3’OH group is absent in ddNTP’s which cause termination of growing DNA chain during Sanger’s DNA sequencing method. DNA fragments formed by chain termination in all the four tubes for the given strand 3’ ATGCTAGC 5' |
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