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Why Is It Necessary To Degass The Mobile Phase? |
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Answer» Mobile PHASES entrap air from the ATMOSPHERE and this trapped air gets released as small bubbles under high pressures encountered during the HPLC analysis. Such bubbles can lead to noise in DETECTOR response or hinder flow of mobile PHASE through columns. In order to overcome such problems degassing of mobile phase becomes ESSENTIAL. Mobile phases entrap air from the atmosphere and this trapped air gets released as small bubbles under high pressures encountered during the HPLC analysis. Such bubbles can lead to noise in detector response or hinder flow of mobile phase through columns. In order to overcome such problems degassing of mobile phase becomes essential. |
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