Why should spotting of mixture be done above the level of mobile phase – InterviewSolution

1.

Why should spotting of mixture be done above the level of mobile phase ?

Answer»

If spotting of a mixture is done at the level of mobile phase, then solvent will come in contact with the sample spot.
Sample spot will dissolve in the mobile phase and its components will move all over the plate resulting in no distinct separation.

Hence,

Spotting of mixture should be done above the level of mobile phase.

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