1.

Write down the procedure and result of Hematoxylin and eosin staining method.

Answer»

1. Fix the smears in 95% alcohol for 30 min 

2. Wash in running tap water 

3. Stain in hematoxylin for 5 min 

4. Wash in running tap water 

5. Decolorize in acid alcohol and wash in running tap water 

6. Ammonia water – 1 dip and then wash in tap water 

7. Stain in eosin for 2 min 

8. Dip in 100% alcohol for 2 min 

9. Dip in 100% alcohol for 2 min 

10. Dip in 100% alcohol for 2 min 

11. Dip in acetone for 2 min 

12. Dehydrate and mount with DPX

Result: 

Nuclei: Blue/Black 

Cytoplasm: Varying shades of pink



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