Saved Bookmarks
| 1. |
Write down the procedure and result of Hematoxylin and eosin staining method. |
|
Answer» 1. Fix the smears in 95% alcohol for 30 min 2. Wash in running tap water 3. Stain in hematoxylin for 5 min 4. Wash in running tap water 5. Decolorize in acid alcohol and wash in running tap water 6. Ammonia water – 1 dip and then wash in tap water 7. Stain in eosin for 2 min 8. Dip in 100% alcohol for 2 min 9. Dip in 100% alcohol for 2 min 10. Dip in 100% alcohol for 2 min 11. Dip in acetone for 2 min 12. Dehydrate and mount with DPX Result: Nuclei: Blue/Black Cytoplasm: Varying shades of pink |
|