Write the steps you would suggest to be undertaken to obtain a foreign

1.	Write the steps you would suggest to be undertaken to obtain a foreigngene-product.
Answer» Recombinant DNA technology involves the following steps: i. Isolation of DNA ii. Fragmentation of DNA by restriction endonucleases. iii. Isolation of a desired DNA fragment. iv. Amplification of the gene of interest. v. Ligation of the DNA fragment into a vector. vi. Insertion of recombinant DNA into the host. vii. Culturing the host cells on a suitable medium at a large scale. viii. Extraction of the desired gene product. ix. Downstream processing of the products as finished product, ready for marketing.

Write the steps you would suggest to be undertaken to obtain a foreigngene-product.

Answer»

Recombinant DNA technology involves the following steps:

i. Isolation of DNA

ii. Fragmentation of DNA by restriction endonucleases.

iii. Isolation of a desired DNA fragment.

iv. Amplification of the gene of interest.

v. Ligation of the DNA fragment into a vector.

vi. Insertion of recombinant DNA into the host.

vii. Culturing the host cells on a suitable medium at a large scale.

viii. Extraction of the desired gene product.

ix. Downstream processing of the products as finished product, ready for marketing.

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