30 + Interview Questions in Amino Acid Oxidation And The Production Of Urea in Biochemistry Page 1 InterviewSolution

1.	The first enzyme in the pathway carbamoyl phosphate synthase I, is allosterically activated by ___________(a) N-acetylglutamate(b) Acetyl coA(c) Glutamate(d) Carbamoyl phosphateThis question was posed to me in an interview for job.This key question is from Nitrogen Excretion and the Urea Cycle topic in section Amino Acid Oxidation and the Production of Urea of Biochemistry
Answer» The correct OPTION is (a) N-acetylglutamate Explanation: The first ENZYME in the pathway carbamoyl PHOSPHATE SYNTHASE I, is allosterically activated by N-acetylglutamate.

Discussion

2.	Which is the first step in the catabolism of most L-amino acids once they have reached the liver is promoted?(a) Amino transferases(b) Glutaminase(c) Glutamine synthase(d) Glutamate dehydrogenaseI had been asked this question during an online interview.I want to ask this question from Metabolic Fates of Amino Groups topic in chapter Amino Acid Oxidation and the Production of Urea of Biochemistry
Answer» Correct choice is (a) Amino transferases The BEST I can EXPLAIN: The FIRST STEP in the catabolism of most L-amino acids once they have reached the liver is removal of the α-amino groups PROMOTED by enzymes called amino transferases or trasaminases.

Discussion

3.	Urea cycle converts ___________(a) Keto acids into amino acids(b) Amino acids into keto acids(c) Ammonia into a less toxic form(d) Ammonia into a more toxic formI got this question in exam.I would like to ask this question from Nitrogen Excretion and the Urea Cycle in chapter Amino Acid Oxidation and the Production of Urea of Biochemistry
Answer» RIGHT answer is (c) AMMONIA into a less toxic form To elaborate: A less toxic ammonia form is produced by UREA CYCLE.

Discussion

4.	A second amino group is transferred to cituilline from ___________(a) Aspartate(b) Glutamate(c) Alanine(d) GuanineThis question was addressed to me in homework.Question is taken from Nitrogen Excretion and the Urea Cycle in section Amino Acid Oxidation and the Production of Urea of Biochemistry
Answer» The correct OPTION is (a) Aspartate To ELABORATE: The second AMINO group enters from aspartate GENERATED in mitochondria by transamination and transported into the CYTOSOL.

Discussion

5.	Which of the following operates at an important intersection of carbon and nitrogen metabolism?(a) Amino transferases(b) Glutaminase(c) Glutamine synthase(d) Glutamate dehydrogenaseThis question was posed to me during an interview for a job.My enquiry is from Metabolic Fates of Amino Groups in chapter Amino Acid Oxidation and the Production of Urea of Biochemistry
Answer» The CORRECT option is (d) Glutamate dehydrogenase The best I can explain: Glutamate dehydrogenase operates at an important INTERSECTION of CARBON and NITROGEN METABOLISM.

Discussion

6.	Pyridoxal phosphate and its aminate form, pyridoxamine phosphate are tightly bound coenzymes of ___________(a) Amino transferases(b) Glutaminase(c) Glutamine synthase(d) Glutamate dehydrogenaseI have been asked this question in homework.This key question is from Metabolic Fates of Amino Groups in division Amino Acid Oxidation and the Production of Urea of Biochemistry
Answer» Correct OPTION is (a) AMINO transferases For explanation: All aminotransferases have PYRIDOXAL phosphate (PLP) as cofactor.

Discussion

7.	Which is the first amino group entering into urea cycle?(a) carbamoyl phosphate(b) Ornithine(c) Cituilline(d) ArgininosuccinateI have been asked this question in homework.My question is based upon Nitrogen Excretion and the Urea Cycle topic in portion Amino Acid Oxidation and the Production of Urea of Biochemistry
Answer» CORRECT option is (a) carbamoyl PHOSPHATE The best EXPLANATION: The carbamoyl phosphate, which functions as an activated carbamoyl group donor ENTERS the UREA cycle.

Discussion

8.	Trypsinogen is converted to its active state by ___________(a) Enteropeptidase(b) Glutaminase(c) Glutamine synthase(d) Glutamate dehydrogenaseThis question was posed to me during an internship interview.My enquiry is from Metabolic Fates of Amino Groups in division Amino Acid Oxidation and the Production of Urea of Biochemistry
Answer» Right OPTION is (a) Enteropeptidase Easiest explanation: TRYPSINOGEN is converted to its ACTIVE state by enteropeptidase, a proteolytic ENZYME secreted by INTESTINAL cells.

Discussion

9.	Which of the following yields acetyl coA directly?(a) Phenylalanine(b) Isoleucine(c) Lysine(d) AlanineI have been asked this question in exam.This is a very interesting question from Pathways of Amino Acid Degradation in section Amino Acid Oxidation and the Production of Urea of Biochemistry
Answer» The CORRECT option is (b) Isoleucine Best EXPLANATION: Isoleucine, leucine, threonine and tryptophan yield acetyl COA DIRECTLY.

Discussion

10.	Which of the following yields acetyl co-A via aceto-acetyl co-A?(a) Leucine(b) Isoleucine(c) Threonine(d) AlanineI had been asked this question in an interview for internship.Question is from Pathways of Amino Acid Degradation in division Amino Acid Oxidation and the Production of Urea of Biochemistry
Answer» Right choice is (a) Leucine The best I can EXPLAIN: Leucine, lysine, PHENYLALANINE and tryptophan yield ACETYL co-A VIA aceto-acetyl co-A.

Discussion

11.	Which of the following produces oxaloacetate?(a) Aspartate(b) Isoleucine(c) Threonine(d) AlanineThe question was posed to me in an interview.The doubt is from Pathways of Amino Acid Degradation topic in chapter Amino Acid Oxidation and the Production of Urea of Biochemistry
Answer» RIGHT answer is (a) ASPARTATE Explanation: ASPARAGINE and aspartate PRODUCE oxaloacetate.

Discussion

12.	Fumarate is produced from four carbon atoms of _________(a) Tyrosine(b) Isoleucine(c) Threonine(d) AlanineThe question was posed to me in an interview for internship.I'd like to ask this question from Pathways of Amino Acid Degradation topic in section Amino Acid Oxidation and the Production of Urea of Biochemistry
Answer» RIGHT ANSWER is (a) Tyrosine Best explanation: Four carbon ATOMS of PHENYLALANINE and tyrosine give rise to FUMARATE.

Discussion

13.	The combined action of aminotransferase and glutamate dehydrogenase is referred as ___________(a) Oxidative deamination(b) Transamination(c) Reductive deamination(d) TransdeaminationThe question was asked in an internship interview.Question is taken from Metabolic Fates of Amino Groups in chapter Amino Acid Oxidation and the Production of Urea of Biochemistry
Answer» The correct choice is (d) TRANSDEAMINATION To ELABORATE: The COMBINED action of aminotransferase and glutamate DEHYDROGENASE is REFERRED as transdeamination.

Discussion

14.	The branched chain amino acid is not _________(a) Leucine(b) Isoleucine(c) Valine(d) AlanineThis question was posed to me in an interview for internship.Asked question is from Pathways of Amino Acid Degradation topic in portion Amino Acid Oxidation and the Production of Urea of Biochemistry
Answer» CORRECT option is (d) Alanine Best explanation: Isoleucine, leucine and VALINE are the BRANCHED chain AMINO ACIDS.

Discussion

15.	Glutamine is converted to glutamate and NH4^+ by ___________(a) Amino transferases(b) Glutaminase(c) Glutamine synthase(d) Glutamate dehydrogenaseThis question was addressed to me in a national level competition.My question comes from Metabolic Fates of Amino Groups topic in division Amino Acid Oxidation and the Production of Urea of Biochemistry
Answer» Right choice is (b) Glutaminase Easy EXPLANATION: In the tissues of intestine, liver and KIDNEYS, the amide nitrogen is released as ammonium ion in the MITOCHONDRIA, where glutaminase converts glutamine to GLUTAMATE and NH4^+.

Discussion

16.	Which of the following produces pyruvate?(a) Leucine(b) Isoleucine(c) Lysine(d) AlanineI had been asked this question in homework.My doubt is from Pathways of Amino Acid Degradation in portion Amino Acid Oxidation and the Production of Urea of Biochemistry
Answer» Right option is (d) ALANINE Easiest explanation: The AMINO acids producing pyruvate are alanine, cysteine, glycine, SERINE, threonine and tryptophan.

Discussion

17.	Urea production occurs almost exclusively in ___________(a) Kidneys(b) Liver(c) Blood(d) UrineThis question was posed to me in my homework.The above asked question is from Nitrogen Excretion and the Urea Cycle in section Amino Acid Oxidation and the Production of Urea of Biochemistry
Answer» Right answer is (b) Liver To elaborate: UREA production occurs ALMOST EXCLUSIVELY in the liver and is the fate of most of the ammonia channeled there. The urea passes into the blood stream and thus to the kidneys and is excreted into the URINE.

Discussion

18.	What are the products of urea cycle?(a) One molecule of urea, one molecule of ammonia, one molecule of ATP and one molecule of fumaric acid(b) One molecule of urea, one molecule of AMP, two molecules of ADP and one molecule of fumaric acid(c) One molecule of aspartic acid, one molecule of ammonia, one molecule of ATP and one molecule of fumaric acid(d) Two molecules of urea, two molecules of ammonia, one molecule of ATP and one molecule of fumaric acidI got this question by my school principal while I was bunking the class.This is a very interesting question from Nitrogen Excretion and the Urea Cycle in chapter Amino Acid Oxidation and the Production of Urea of Biochemistry
Answer» RIGHT CHOICE is (b) ONE molecule of urea, one molecule of AMP, two molecules of ADP and one molecule of fumaric ACID Explanation: Products of urea cycle are one molecule of urea, one molecule of AMP, two molecules of ADP and one molecule of fumaric acid.

Discussion

19.	Free ammonia combined with glutamate to yield glutamine by the action of ___________(a) Glutaminase(b) Glutamine synthase(c) Glutamate dehydrogenase(d) Amino transferaseThis question was posed to me in an international level competition.My enquiry is from Metabolic Fates of Amino Groups topic in division Amino Acid Oxidation and the Production of Urea of Biochemistry
Answer» The CORRECT option is (b) Glutamine synthase Easiest explanation: The free ammonia is COMBINED with glutamate to YIELD glutamine by the action of glutamine synthase.

Discussion

20.	Which of the following produces α-ketoglutarate?(a) Leucine(b) Threonine(c) Methionine(d) ProlineThe question was asked in exam.My question is taken from Pathways of Amino Acid Degradation in chapter Amino Acid Oxidation and the Production of Urea of Biochemistry
Answer» Right ANSWER is (d) Proline For EXPLANATION: Arginine, GLUTAMATE, glutamine, HISTIDINE and proline produce α-ketoglutarate.

Discussion

21.	Which of the following amino acids are exclusively ketogenic?(a) Leucine(b) Asparagine(c) Threonine(d) ProlineThis question was addressed to me in a job interview.I'm obligated to ask this question of Pathways of Amino Acid Degradation topic in section Amino Acid Oxidation and the Production of Urea of Biochemistry
Answer» Right OPTION is (a) Leucine For explanation I WOULD SAY: Only two amino acids, leucine and LYSINE are exclusively KETOGENIC.

Discussion

22.	What is the necessary coenzyme for transamination reactions?(a) Pyridoxal phosphate(b) Thiamine pyrophosphate(c) NAD(d) Coenzyme AI had been asked this question by my college professor while I was bunking the class.This intriguing question originated from Metabolic Fates of Amino Groups in chapter Amino Acid Oxidation and the Production of Urea of Biochemistry
Answer» RIGHT choice is (a) Pyridoxal phosphate For explanation I WOULD say: Pyridoxal phosphate functions as an intermediate carrier of amino GROUPS at the active site of amino TRANSFERASES.

Discussion

23.	Glutamate is metabolically converted to α-ketoglutarate and NH4^+ by a process ___________(a) Oxidative deamination(b) Transamination(c) Reductive deamination(d) DeaminationThis question was posed to me during a job interview.I'd like to ask this question from Metabolic Fates of Amino Groups topic in portion Amino Acid Oxidation and the Production of Urea of Biochemistry
Answer» Correct option is (a) OXIDATIVE deamination To explain I WOULD SAY: Glutamate is metabolically converted to α-ketoglutarate and NH4^+ by a process oxidative deamination CATALYZED by L-glutamate dehydrogenase.

Discussion

24.	Which of the following is not an essential amino acid?(a) Glycine(b) Leucine(c) Methionine(d) HistidineThis question was addressed to me in an interview.This key question is from Nitrogen Excretion and the Urea Cycle topic in chapter Amino Acid Oxidation and the Production of Urea of Biochemistry
Answer» The correct OPTION is (a) Glycine For EXPLANATION: Only LEUCINE, methionine and histidine are essential in this case.

Discussion

25.	Which of the following are exclusively glucogenic?(a) Arginine(b) Leucine(c) Lysine(d) ThreonineThis question was addressed to me in semester exam.This interesting question is from Pathways of Amino Acid Degradation topic in division Amino Acid Oxidation and the Production of Urea of Biochemistry
Answer» CORRECT choice is (a) Arginine To EXPLAIN: Leucine and LYSINE are exclusively KETOGENIC. Threonine is both ketogenic and GLUCOGENIC.

Discussion

26.	Nitrogen atoms of urea produced in the urea cycle are derived from ___________(a) Ammonia and aspartic acid(b) Nitrate(c) Nitrite(d) AmmoniaThis question was addressed to me by my college director while I was bunking the class.My doubt is from Nitrogen Excretion and the Urea Cycle topic in section Amino Acid Oxidation and the Production of Urea of Biochemistry
Answer» CORRECT option is (a) Ammonia and aspartic acid The explanation: One AMINO group COMES from ammonia and ANOTHER comes from aspartate.

Discussion

27.	Which of the following produce succinyl co-A?(a) Leucine(b) Isoleucine(c) Arginine(d) AlanineI got this question by my school teacher while I was bunking the class.I need to ask this question from Pathways of Amino Acid Degradation in chapter Amino Acid Oxidation and the Production of Urea of Biochemistry
Answer» The CORRECT choice is (B) Isoleucine The explanation: Isoleucine, methionine, threonine and valine PRODUCE SUCCINYL co-A.

Discussion

28.	What are the inputs to one cycle of urea cycle?(a) One molecule of CO2, one molecule of ammonia, three molecules of ATP and one molecule of aspartic acid(b) One molecule of urea, one molecule of AMP, two molecules of ADP and one molecule of fumaric acid(c) One molecule of aspartic acid, one molecule of ammonia, one molecule of ATP and one molecule of fumaric acid(d) Two molecules of urea, two molecules of ammonia, one molecule of ATP and one molecule of fumaric acidThe question was asked in examination.Enquiry is from Nitrogen Excretion and the Urea Cycle topic in division Amino Acid Oxidation and the Production of Urea of Biochemistry
Answer» Correct choice is (a) One molecule of CO2, one molecule of ammonia, three MOLECULES of ATP and one molecule of aspartic acid For explanation I would say: One molecule of CO2, one molecule of ammonia, three molecules of ATP and one molecule of aspartic acid are the INPUTS to one CYCLE of urea cycle.

Discussion

29.	The carbon atom source while producing urea in the urea cycle is ___________(a) CO2(b) Glucose(c) Aspartic acid(d) ArginineThis question was posed to me in an international level competition.My question comes from Nitrogen Excretion and the Urea Cycle in division Amino Acid Oxidation and the Production of Urea of Biochemistry
Answer» CORRECT OPTION is (a) CO2 Easiest EXPLANATION: CO2 is the carbon atom source while PRODUCING urea in the urea cycle.

Discussion

30.	Which of the following hydrolyzes successive amino-terminal residues from short peptides?(a) Aminopeptidase(b) Enteropeptidase(c) Glutamine synthase(d) Glutamate dehydrogenaseThe question was asked in class test.My question is based upon Metabolic Fates of Amino Groups in division Amino Acid Oxidation and the Production of Urea of Biochemistry
Answer» The CORRECT CHOICE is (a) Aminopeptidase Easiest EXPLANATION: Aminopeptidase hydrolyzes successive amino-terminal residues from SHORT PEPTIDES.

Discussion

Explore topic-wise InterviewSolutions in .

A second amino group is transferred to cituilline from ___________(a) Aspartate(b) Glutamate(c) Alanine(d) GuanineThis question was addressed to me in homework.Question is taken from Nitrogen Excretion and the Urea Cycle in section Amino Acid Oxidation and the Production of Urea of Biochemistry

Which of the following yields acetyl coA directly?(a) Phenylalanine(b) Isoleucine(c) Lysine(d) AlanineI have been asked this question in exam.This is a very interesting question from Pathways of Amino Acid Degradation in section Amino Acid Oxidation and the Production of Urea of Biochemistry

Which of the following produces oxaloacetate?(a) Aspartate(b) Isoleucine(c) Threonine(d) AlanineThe question was posed to me in an interview.The doubt is from Pathways of Amino Acid Degradation topic in chapter Amino Acid Oxidation and the Production of Urea of Biochemistry

The branched chain amino acid is not _________(a) Leucine(b) Isoleucine(c) Valine(d) AlanineThis question was posed to me in an interview for internship.Asked question is from Pathways of Amino Acid Degradation topic in portion Amino Acid Oxidation and the Production of Urea of Biochemistry

Which of the following produces pyruvate?(a) Leucine(b) Isoleucine(c) Lysine(d) AlanineI had been asked this question in homework.My doubt is from Pathways of Amino Acid Degradation in portion Amino Acid Oxidation and the Production of Urea of Biochemistry

Urea production occurs almost exclusively in ___________(a) Kidneys(b) Liver(c) Blood(d) UrineThis question was posed to me in my homework.The above asked question is from Nitrogen Excretion and the Urea Cycle in section Amino Acid Oxidation and the Production of Urea of Biochemistry

Which of the following produces α-ketoglutarate?(a) Leucine(b) Threonine(c) Methionine(d) ProlineThe question was asked in exam.My question is taken from Pathways of Amino Acid Degradation in chapter Amino Acid Oxidation and the Production of Urea of Biochemistry

Which of the following is not an essential amino acid?(a) Glycine(b) Leucine(c) Methionine(d) HistidineThis question was addressed to me in an interview.This key question is from Nitrogen Excretion and the Urea Cycle topic in chapter Amino Acid Oxidation and the Production of Urea of Biochemistry

Which of the following are exclusively glucogenic?(a) Arginine(b) Leucine(c) Lysine(d) ThreonineThis question was addressed to me in semester exam.This interesting question is from Pathways of Amino Acid Degradation topic in division Amino Acid Oxidation and the Production of Urea of Biochemistry