InterviewSolution
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InterviewSolution
This section includes InterviewSolutions, each offering curated multiple-choice questions to sharpen your knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
The process of separation and purificaition of expressed protein before marketing is calledA. Upstream processingB. Downstream processingC. BioprocessingD. Postproduction processing |
| Answer» Correct Answer - b | |
| 2. |
Assertion: A gene from bacillus thuringiensis is incorporated in plant genome to increases yeild. Reason : it is Bt toxin producing genw which kills larvae of insects.A. If both the assertion and the reason are true and the reason is a correct explanation of the assertionB. If both the assertion and reason are true but the reason is not a correct explanation of the assertionC. IF the assertion is true but the reason is falseD. If both the assertion and reason are false |
| Answer» Correct Answer - a | |
| 3. |
The stired-tank reactor is usuallyA. CylindericalB. RoundedC. Cup-ShapedD. Flattened |
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Answer» Correct Answer - a A stirred-tank bioreactor is usually cylinderical or with a curved base to facilitate the mixing of the reaction contents. The stirrer facilitates even mixing and oxygen availability throughout the bioreactor alternatively air can be bubbled through the reactor. |
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| 4. |
Which one of the following techniques made it possible to gnetically engineer living organisms ?A. Heavier isotope labelingB. HybridizationC. Recombinant DNA techniquesD. X-ray diffraction |
| Answer» Correct Answer - c | |
| 5. |
If recombinant DNA is inserted within the coding sequence of enzyme galactosidase, which of the following will occur in case of non -recombinantsA. Insertional inactivationB. Colonies do not produce any colourC. Chromogenic substrate gives blue colourD. Inactivation of enzyme galactosidase |
| Answer» Correct Answer - c | |
| 6. |
Which one of the following technique made it possible to genetically engineering living organismsA. Heavier isotope labelingB. HybridizationC. Recombinant DNA techniquesD. X-ray diffraction |
| Answer» Correct Answer - c | |
| 7. |
DNA polymerase enzyme is isolated from which bacteriaA. E.ColiB. Thermus aquaticusC. Bacillus thuringenesisD. Agro bacterium |
| Answer» Correct Answer - a | |
| 8. |
The colonies of recombinant bacteria appear white in because ofA. Inactivation of glycosidase enzyme in recombinant bacteriaB. Non-recombinant bacteria containing beta -galactosidaseC. Insertional inactivation of alpha-galactosidase in non-recombinant bacteriaD. Insertional inactivation of alpha-galactosidasea in recombinant bacteria |
| Answer» Correct Answer - c | |
| 9. |
For rapid production of alcohol , immobilised yeast calles are kept inA. Silica gelB. Wire nettingC. Porcelain columnsD. Calcium alginate beads |
| Answer» Correct Answer - d | |
| 10. |
What is the source of EcoRIA. Escherichia coli RIB. Escherichia coli RI 13C. Escherichia coli 13D. Escherichia Coli RY 13 |
| Answer» Correct Answer - d | |
| 11. |
Who among the following was awarded the Nobal Prize for the development of PCR technique ?A. Herbert BoyerB. Hargovind KhuranaC. Kary mullisD. Arthur kornberg |
| Answer» Correct Answer - c | |
| 12. |
Genetically engineered bacteria are being employed for production ofA. ThyroxinB. ProgesteroneC. InsulinD. Estrogen |
| Answer» Correct Answer - c | |
| 13. |
Which of the following steps are catalysed by Taq polymerase in a PCR reaction ?A. Denaturation of template DNAB. Annealing of primers to template DNAC. Extension of primer end of the template DNAD. All of the above |
| Answer» Correct Answer - c | |
| 14. |
The correct order of steps in polymerase chain Reaction (PCR) isA. Extension, Denaturation,AnnealingB. Annealing , Extension,DenaturationC. Denaturation,Extension,AnnealingD. Denaturation,Annealing,Extension |
| Answer» Correct Answer - d | |
| 15. |
The function of a selectable maker isA. Eliminating transormation and permitting non-transformantsB. Identify ori siteC. Elimination of non-transformants and permitting transformantsD. To destroy recongnition sites |
| Answer» Correct Answer - c | |
| 16. |
In recombinant DNA technology, the term vector refers toA. The enzyme that cuts DNA into restriction fragmentsB. the sticky end of DNA fragmentsC. A plasmid used to transger DNA into a living cellD. A DNA probe used to identify a particular gene |
| Answer» Correct Answer - c | |
| 17. |
Restriction endonucleases are most widely used in recombinant DNA technology. They are obtained fromA. BacteriphagesB. Bacterial cellsC. PlasmidsD. All prokaryotic cells |
| Answer» Correct Answer - b | |
| 18. |
In genetic engineering, a DNA segment (gene) of interest, is translated to the host cell through a vector. Consider the following four agents (A-D) in this regard and select the correct option about which one or more of these can be used as a vector/vectors Statement (A) A bacterium (B) Plasmid (C) Plasmodium (D) BacteriophageA. (A),(B) and (D) OnlyB. (A) onlyC. (A) and (C) onlyD. (B) and (C) only |
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Answer» Correct Answer - d Plasmids and bacteriphages are used as vectors in genetic engineering. |
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| 19. |
The following cycle refer to the PCR prcess, name the factors or steps indicated with numbers A. (i) Taq polumerase (ii) Extension (iii) LigationB. (i) Primer (ii) Denaturation at `94^(@)C` (iii) Taq polymeraseC. (i) Denaturation at `94^(@)C` (ii) Taq polymerase (iii) PrimerD. (i) Denaturation at `94^(@)C` (ii) Taq polymerase (iii) Primer |
| Answer» Correct Answer - d | |
| 20. |
Which of the followijng is/are used in recombinant DNA technology A. Agarose gel (B). Ethidium bromide (C) . Plasmid vector (D) Restriction endonucleaseA. A,BB. B,CC. C,DD. A,B,C,D |
| Answer» Correct Answer - d | |
| 21. |
First biochemical to be produced comercially by microbial cloning and genetic engineering isA. Humann insulinB. PenicillinC. InterferonsD. Fertility factors |
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Answer» Correct Answer - a Comercially production of E.coli genetically engineered human insulin |
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| 22. |
Which one among the following is just a cloning plasmid not an expression plasmidA. pBAD-18-CamB. P BCSKC. pUC18D. pET |
| Answer» Correct Answer - c | |
| 23. |
Which of the following organelles is releated with genetic engineering/gene cloningA. Golgi apparatusB. LysosomesC. MitochondriaD. Plasmids |
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Answer» Correct Answer - d Plasmids are extrachromosomal covalently closed circular doubles standard molecules of DNA present in most prokaryotes. Therefore they are used as a vectors in genetic engineering. |
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| 24. |
DNA recombinant technology uses A . Restiction endonucleases B. DNA ligase C. Cloning vector D. ElectrophoresisA. A,BB. B,CC. C,DD. A,B,C,D |
| Answer» Correct Answer - d | |
| 25. |
Which of these is used as vector in gene therapy for SCIDA. ArbovirusB. RotavirusC. EnterovirusD. Paravovirus |
| Answer» Correct Answer - c | |
| 26. |
What is the permanent cure of adenosine deaminase (ADA) deficiency in children ?A. Hybridoma technologyB. Gene therapyC. rDNA technologyD. Embryo transfer |
| Answer» Correct Answer - b | |
| 27. |
The linking of antiobiotic resistance gene with the plasmid vector became possible withA. DNA polymeraseB. ExonucleasesC. DNA ligaseD. Endonucleases |
| Answer» Correct Answer - c | |
| 28. |
Which of these is widely used in genetic engineeringA. AnophelesB. Dragon flyC. Dragon lizardD. Fruit fly |
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Answer» Correct Answer - d Fruit fly also known as drosophilia is widely used cloning vectors, it contains both ampicillin and tetracycline resistance genes. |
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| 29. |
Which vector can clone only a small fragement of DNAA. PlasmidB. CosmidC. Bacterial artificial chromosomesD. Yeast artificial chromosomes |
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Answer» Correct Answer - a Plasmid can clone only a small fragment of DNA about 10 kbp size Cosmid -45 kkbp YAC -1 Mbp/1000 kbp-2,500 kbp BAC -300 to 350 kbp. |
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| 30. |
Which of the following is not required in the preparation of a recombinant DNA molecule ?A. Restriction endonucleaseB. DNA ligaseC. DNA fragmentsD. E coil |
| Answer» Correct Answer - d | |
| 31. |
Which of the following statements does not hold true for restriction enzyme ?A. it recognize a palindromic nucleotide sequenceB. it is an endonucleaseC. it is isolated isolated from virusesD. It can produce the same kind of sticky ends in different DNA molecules |
| Answer» Correct Answer - c | |
| 32. |
An enzyme catalysing the removal of nucleotides from the ends of DNA isA. EndonucleaseB. ExonucleaseC. DNA ligaseD. Hind-II |
| Answer» Correct Answer - b | |
| 33. |
Which of the following is not a component of downstream processingA. ExpressionB. separationC. PurificationD. Preservation |
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Answer» Correct Answer - a Expression of recombinant DNA is parts of upstream processing |
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| 34. |
The figure below is the diagrammatic representation of the E.coli vector pBr 322. which one of the given options correctly identifies its certain component (s) A. Ori-original restriction enzymesB. Ro-reduced osmotic pressureC. Hind III, EcoRI -Selectable markersD. `amp^(R),tet^(R)` -antibiotic resistance genes |
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Answer» Correct Answer - d In pBR322 ori-represents site of origin or replication, rop-represents those proteins that take part in replication of plasmid. Hind III, ECORI-Recoginition sites of Restriction endonucleases, `amp^(R)` and `tet^(R)`-they are antiniotic resistance gene part. |
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| 35. |
Identify the plasmidA. AIU IB. Hind IIIC. Eco RID. pBr 322 |
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Answer» Correct Answer - d Plasmid pBr 322 was one of the first widely used cloning vectors, it contains both ampicllin and tetracycline resistance genes. |
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| 36. |
It is now possible to breed plants and animals with desired characters throughA. Genetic engineeringB. Chromosome engineeringC. lkebana techniqueD. tissue culture |
| Answer» Correct Answer - a | |
| 37. |
In recombination vector used isA. ProteinB. Agrobacterium tumerfaciensC. Nucleic acidD. Cellulose |
| Answer» Correct Answer - b | |
| 38. |
Recombinant DNA is force to enter the host cells by incubating the cells with rDNA firstA. On ice followed by heat shock and then again on iceB. At low temperature followed by heat shock and then on iceC. In ethidium bromide followed by calcium saltsD. Into chilled ethanol followed by ice and then into CsCl |
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Answer» Correct Answer - a The host cells with r-DNA are first subjected to ice followed by heat shoch `(42^(@)C)` and then to ice. As a result, rDNA molcules are easily introduced into the host cells. This process is called transformation. |
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| 39. |
Recombinant DNA (rDNA) tenchnology is related withA. C.darwinB. Stanley CohenC. Herbert BoyerD. Both (b) and (c) |
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Answer» Correct Answer - d The first recombinant DNA was constructed by stanley chen and herbert in 1972. they cut the piece of DNA from from a plasmid carrying antiobiotic resistance gene in the bacterium salmonella typhimurium and linked it to the plasmid of escherichia coli. |
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