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51.

Southern blotting technique is used in ____________(a) Monoclonal antibody production(b) In vitro culture(c) Genetic finger printing(d) Polymerase chain reactionI got this question during an interview for a job.Origin of the question is From Genes to Genomes in section DNA-Based Information Technologies of Biochemistry

Answer»

Right option is (C) GENETIC finger printing

The explanation is: APPLICATIONS of southern blotting:

In RFLP, FORENSICS, diagnosis of HIV, etc.

52.

How are expression vectors useful?(a) To produce protein products(b) Used for genomic libraries(c) Used for chromosome synthesis(d) Used for finger printingThe question was asked in an interview for internship.Question is taken from From Genes to Genomes topic in chapter DNA-Based Information Technologies of Biochemistry

Answer»

Right choice is (a) To PRODUCE PROTEIN products

For EXPLANATION: EXPRESSION vectors are helpful for the expression of GENES.

53.

Which of the following is the correct order of organization of genetic material from largest to smallest?(a) Genome, chromosome, gene, nucleotide(b) Nucleotide, gene, chromosome, genome(c) Gene, nucleotide, chromosome, genome(d) Chromosome, genome, nucleotide, geneI got this question in a national level competition.My enquiry is from From Genes to Genomes-1 topic in division DNA-Based Information Technologies of Biochemistry

Answer»

The CORRECT option is (a) Genome, chromosome, gene, nucleotide

The BEST explanation: GROUP of nucleotides makes up a gene, these GENES make up a chromosome and these chromosomes make up to genome.

54.

The extra chromosomal, self-replicating, closed, double stranded and circular DNA molecule is generally termed as __________(a) Chromosome(b) Plasmid(c) Genomic DNA(d) BacteriophageI got this question in unit test.My question is based upon DNA Cloning: The Basics-1 topic in chapter DNA-Based Information Technologies of Biochemistry

Answer»

The CORRECT option is (b) Plasmid

To EXPLAIN I would SAY: Plasmid is an extra chromosomal, self-replicating, closed, double STRANDED and circular DNA molecule.

55.

Dolly, the first animal produced by cloning is a __________(a) Cow(b) Sheep(c) Rat(d) DogThe question was asked in a national level competition.The origin of the question is Genome Alterations and New Products of Biotechnology in section DNA-Based Information Technologies of Biochemistry

Answer» RIGHT option is (b) SHEEP

Explanation: Dolly is a female domestic sheep that was cloned using nuclear TRANSFER process from an ADULT somatic cell.
56.

In hybridization method, which of the following is pressed onto agar?(a) Silicon paper(b) Nitrocellulose paper(c) Filter paper(d) PVDFI had been asked this question at a job interview.The query is from DNA Cloning: The Basics in section DNA-Based Information Technologies of Biochemistry

Answer» RIGHT ANSWER is (B) Nitrocellulose paper

For EXPLANATION I would say: Nitrocellulose MEMBRANE has high binding capacity for proteins.
57.

DNA Ligase, used in recombinant DNA technology is obtained from _______________(a) E.coli only(b) E.coli and also Ligase encoded by T4 phage(c) Saccharomyces(d) RetrovirusesI had been asked this question during an online interview.I want to ask this question from DNA Cloning: The Basics in portion DNA-Based Information Technologies of Biochemistry

Answer» RIGHT ANSWER is (b) E.coli and also Ligase encoded by T4 phage

Best explanation: Ligase obtained from Ecoli and T4 DNA ligase is used in RECOMBINANT DNA TECHNOLOGY.
58.

Which of the following statements is false?(a) The cells which take up recombinant plasmids are drug resistant(b) The cells with no recombinant plasmids are drug resistant(c) Plasmid replication is independent of cell’s division(d) The cells containing recombinant plasmid are called transformed cellsThis question was posed to me in semester exam.My query is from DNA Cloning: The Basics-1 in portion DNA-Based Information Technologies of Biochemistry

Answer»

The CORRECT choice is (a) The cells which take up RECOMBINANT plasmids are drug RESISTANT

To explain: Recombinant plasmid contains a drug resistant GENE.

59.

Which of the following enzymes’ combined action leads to the generation of sticky ends in the plasmid vector?(a) Alkaline phosphatase and terminal transferase(b) Exonuclease III and alkaline phosphatase(c) Bacteriophage lambda exonuclease and terminal transferase(d) Exonuclease III and terminal transferaseI had been asked this question in an interview.The doubt is from DNA Cloning: The Basics-1 in portion DNA-Based Information Technologies of Biochemistry

Answer»

Correct OPTION is (c) BACTERIOPHAGE LAMBDA exonuclease and terminal transferase

To elaborate: The combined action of bacteriophage lambda exonuclease and terminal transferase

lead to the generation of sticky ends in plasmid vector.

60.

Which of the following enzyme is responsible for making a DNA copy from RNA?(a) Reverse transcriptase(b) DNA polymerase(c) RNA polI(d) RNA polIII had been asked this question in semester exam.Origin of the question is DNA Cloning: The Basics-1 in section DNA-Based Information Technologies of Biochemistry

Answer»

The correct choice is (a) Reverse TRANSCRIPTASE

Best EXPLANATION: Reverse transcriptase GENERATES complementary DNA from RNA TEMPLATE.

61.

Which of the following bacterium is used for gene transfer in plants?(a) Agrobacterium(b) Azatobacter(c) Rhizobium(d) EcoliI got this question during an internship interview.This intriguing question comes from Genome Alterations and New Products of Biotechnology topic in division DNA-Based Information Technologies of Biochemistry

Answer» RIGHT choice is (a) Agrobacterium

Easiest EXPLANATION: Agrobacterium is the only bacterium CAPABLE of transferring GENES to PLANTS.
62.

Which of the following is used to transfer genes in plants?(a) Ti plasmid(b) pBR 322(c) EcorI(d) pUC 18I had been asked this question in unit test.I need to ask this question from Genome Alterations and New Products of Biotechnology topic in chapter DNA-Based Information Technologies of Biochemistry

Answer»

Right OPTION is (a) Ti PLASMID

Easiest EXPLANATION: Ti plasmid of agrobacterium is used to TRANSFER genes in plants.

63.

Transgenic organisms are generally __________(a) Extinct organisms(b) Naturally occurring and endemic(c) Produced by traditional plant breeding technique(d) Produced by gene transfer technologyI had been asked this question in an interview for job.I need to ask this question from Genome Alterations and New Products of Biotechnology in chapter DNA-Based Information Technologies of Biochemistry

Answer» CORRECT choice is (d) Produced by gene transfer technology

Easy explanation: TRANSGENIC technology, ALSO known as genetic engineering involves the transfer of genes from one SPECIES to another.
64.

Glucose is added to the tissue culture media as a/an __________(a) Carbon source(b) Growth regulator(c) Solidifying agent(d) AntibioticThe question was asked in semester exam.Enquiry is from Genome Alterations and New Products of Biotechnology in portion DNA-Based Information Technologies of Biochemistry

Answer»

Right CHOICE is (a) Carbon source

The explanation: Energy is DERIVED from GLUCOSE.

65.

Which of the following is true about plasmids?(a) They are present in all organisms(b) They are present in bacteria only(c) They are present in bacteria and phages(d) They are present in plants and animalsI have been asked this question during an interview for a job.My question is based upon DNA Cloning: The Basics in portion DNA-Based Information Technologies of Biochemistry

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The correct ANSWER is (C) They are PRESENT in BACTERIA and phages

For explanation: Plasmids cannot be found in ANIMAL cells. They are found only in bacteria and phages.

66.

Short DNA sequence having single occurrence in genome is ____________(a) Expressed sequence tag(b) Sequence tagged site(c) Contig(d) YACThe question was posed to me in an online quiz.This interesting question is from From Genes to Genomes-1 in division DNA-Based Information Technologies of Biochemistry

Answer»

Right CHOICE is (b) Sequence tagged site

Explanation: Expressed sequence tag is a short sub-sequence of a cDNA sequence.

Cotig is a SET of overlapping DNA SEGMENTS that together REPRESENT a consensus region of DNA.

YAC is a linear DNA molecule.

67.

Which of the following are the two methods of screening?(a) Hybridization and PCR(b) ELISA and blotting(c) ELISA and PCR(d) PCR and RFLPI had been asked this question in final exam.My enquiry is from From Genes to Genomes-1 topic in division DNA-Based Information Technologies of Biochemistry

Answer»

Correct OPTION is (a) Hybridization and PCR

For EXPLANATION: Hybridization and PCR are the two METHODS of SCREENING.

68.

The work ‘Hybridization’ in DNA finger printing means ____________(a) Pairing between the nucleotides of DNA sample with probe(b) Pairing between the nucleotides of DNA and mRNA(c) Pairing between the nucleotides of probe with mRNA(d) Pairing between the nucleosides with mRNAThis question was posed to me in class test.The query is from DNA Cloning: The Basics topic in portion DNA-Based Information Technologies of Biochemistry

Answer»

Right answer is (a) Pairing between the nucleotides of DNA SAMPLE with probe

The BEST EXPLANATION: Hybridization involves annealing of DNA segment with its probe.

69.

Repressor molecules bind to __________(a) Operator(b) Promoter(c) Enhancer(d) Hormone response elementThe question was posed to me during an interview.My doubt stems from DNA Cloning: The Basics-1 topic in section DNA-Based Information Technologies of Biochemistry

Answer» CORRECT option is (a) Operator

The best I can EXPLAIN: An operator lies partially within the PROMOTER, repressor binds to the DNA at operator region INHIBITING the EXPRESSION of genes.
70.

What is the main aim of human genome project?(a) To identify and sequence of all the genes present in the human body(b) To introduce new genes to human beings(c) To remove disease causing genes from humans(d) To improve techniques of finger printingI got this question in semester exam.The origin of the question is DNA Cloning: The Basics in chapter DNA-Based Information Technologies of Biochemistry

Answer»

The correct answer is (a) To identify and sequence of all the GENES present in the human body

The BEST I can explain: The main AIM of human GENOME PROJECT is to identify and sequence of all the genes present in the human body.