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1.	Products of restriction enzyme digestion are separated byA. Agarose gel electrophoresisB. CentrifugationC. Polyacrylamide gel electrophoresisD. PCR
Answer» Correct Answer - A

2.	Bacillus thuringiensis strains have been used in designing novelA. BiofertilizersB. Bioinsecticidal plantsC. Biomineralization processD. Biometallugical techniques
Answer» Correct Answer - B

3.	In the three steps (a,b,c) of polymerase chain reaction, select the correct step A. c- extension in presence of heat stable DNA polymeraseB. a - annealing with two sets of primersC. b - denaturation at high temperatureD. a - denaturation at `50^(@)C`
Answer» Correct Answer - D

4.	Cohen and Boyer isolated an antibiotic resistance gene, by cutting out a piece of DNA from a plasmid which was responsible for conferring antibiotic resistance, in the yearA. 1962B. 1965C. 1972D. 1982
Answer» Correct Answer - C

5.	Sticky ends of two DNA strands are jointed by the action of enzymeA. DNA ligaseB. EndonucleaseC. ExonucleaseD. DNA polymerase
Answer» Correct Answer - A

6.	DNA fragments with sticky ends are not allowed to undergo self ligation byA. UnwindaseB. Single strand binding proteinsC. GyraseD. Alkaline phosphatase
Answer» Correct Answer - D

7.	Flavr Savr Tomato has increasedA. ProductivityB. VigourC. Shelf lifeD. Flowering period
Answer» Correct Answer - C

8.	Restriction enzyme (s) of recombinant DNA technology that make staggered cuts leaving stickly ends is/areA. EcoRIB. Hind IIIC. Bam HID. All the above
Answer» Correct Answer - D

9.	Which vector is used to replace defective gene in gene therapyA. CosmidB. Ri plasmidC. Ti plasmidD. Adenovirus
Answer» Correct Answer - D

10.	SCID is caused by defective gene coding for enzymeA. Adenosine deaminaseB. Adensoine transaminaseC. Adenosine transferaseD. Guanosine transaminase
Answer» Correct Answer - A

11.	GMOs areA. Transgenic microbesB. Transgenic plantsC. Transgenic animalsD. All the above
Answer» Correct Answer - D

12.	Enzyme Taq polymerase is obtained fromA. Thermus aquaticusB. Trichoderma aquaticaC. Tremetes aquaticusD. All the above
Answer» Correct Answer - A

13.	Assertion : Restriction enzymes cut the strand of DNA to produce sticky ends. Reason : Stickness of the ends facilitates the action of the enzyme DNA polymeraseA. if both are true with reason being correct explanationB. both true but reason is not correct explanationC. assertion true but reason is wrongD. both are wrong
Answer» Correct Answer - C

14.	Assertion : Flavr-Savr tomato was the first transgenic commercial crop that entered the market Reason : Roundup variety of Soay Bean was prepared through breeding.A. if both are true with reason being correct explanationB. both true but reason is not correct explanationC. assertion true but reason is wrongD. both are wrong
Answer» Correct Answer - C

15.	Match the columns of substances and enzymes required to degrade them A. a-iv, b-iii, c-i, d-iiB. a-ii, b-i, c-iv, d-iiiC. a-i, b-ii, c-iii, d-ivD. a-iii, b-iv, c-ii, d-i
Answer» Correct Answer - A

16.	Curative technique involving repairing or replacement of defective genes isA. Gene therapyB. Gene replacement therapyC. Antisense therapyD. Sense therapy
Answer» Correct Answer - A