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101.

PCR technique was invented byA. BoyerB. Karry MullisC. CohenD. Sanger

Answer» Correct Answer - B
102.

Xenograft is a graftA. Between genetically identical individualsB. Between individuals of different speciesC. From one individual to another of same speciesD. From one area to another of same individual

Answer» Correct Answer - B
103.

"World Intellectual Property Day" is observed onA. February 29thB. April 26thC. June 30thD. September 5th

Answer» Correct Answer - B
104.

Hirudin gene inserted in Brassica napous wasA. Synthesised chemicallyB. Obtained from leechC. Got from Bacillus thuringiensisD. Brassica campestris.

Answer» Correct Answer - A
105.

Which kind of therapy was given in 1990 to a four year old girl with adenosine deaminase deficiencyA. Radiation therapyB. Gene therapyC. ChemotherapyD. Immunotherapy

Answer» Correct Answer - B
106.

Toxic component of Bacillus thuringiensis isA. AlkaloidB. SteroidC. Amino acidD. Protein

Answer» Correct Answer - D
107.

Which of the following is not a component of downstream processingA. ExpressionB. SeparationC. PurificationD. Presentation

Answer» Correct Answer - A
108.

Increased flavourful shelf life of Tomato has been achieved byA. Developing better storage techniqueB. Reducing activity of enzyme polygalactourinaseC. Promoting activity of enzyme polygalactourinaseD. Enhancing epidermal growth factor.

Answer» Correct Answer - B
109.

Flavr savr variety of Tomato is improved variety developed throughA. Hybridisation between old varietiesB. Hybridisation between a modern variety and a wild varietyC. Mutation varietyD. Incorporation of a transgene

Answer» Correct Answer - D
110.

Which can be used as vector for transfer of genesA. BACB. YACC. PlasmidD. All the above

Answer» Correct Answer - D
111.

Blunt ends of passenger and vehicle DNAs are joined byA. DNA polymerase IB. RNA polymeraseC. DNA ligaseD. DNA polymerase III

Answer» Correct Answer - C
112.

Select the matched oneA. Biolists - biorectorB. Thermus aquaticus - T-DNAC. Plasmid DNA - vectorD. EcoRI - restriction exonuclease

Answer» Correct Answer - C
113.

Which is correctly matchedA. Central dogma - codonB. RNA polymerase - RNA primerC. Okazaki fragments - splicingD. Restriction enzyme - genetic engineering

Answer» Correct Answer - D
114.

Which is wrongly matchedA. Somatic hybridisation - Fusion of two diverse cellsB. Vector DNA-Site for tRNA synthesisC. Micropropagation-In vitro production of plants in large numberD. Callus-Unorganised mass of produced in tissue culture.

Answer» Correct Answer - B
115.

A patient brought to a hospital with myocardial infraction is normally immediately givenA. StreptokinaseB. Cyclospoirin -AC. StatinsD. Penicillin

Answer» Correct Answer - A
116.

Which can be used as vector for transfer of DNA segment (a) bacterium, (b) plasmid (c) plasmodium , (d) bacteriophageA. a, b and dB. a onlyC. a and cD. b and d

Answer» Correct Answer - D
117.

Escherichia coli isA. Gut bacteriumB. Soil bacteriumC. Thermal bacteriumD. Both A and B

Answer» Correct Answer - A
118.

The problem of blindness in poor countries can be taken care of by using the following:A. Golden RiceB. Transgenic MaizeC. Transgenic TomatoD. Bt Brinjal

Answer» Correct Answer - A
119.

GMO technology is useful forA. Making crops more tolerant to abiotic stressesB. Helping to reduce post-harvest lossesC. Enhancing nutritional value of foodD. All the above

Answer» Correct Answer - D
120.

A genetic disorder can be cured throughA. Gene therapyB. rDNA technologyC. Embryo transferD. Hybridisation technology

Answer» Correct Answer - A
121.

Cells obtained from cancerous tumours are known asA. HybridomaB. LymphocyteC. Monoclonal cellsD. Myeloma

Answer» Correct Answer - D
122.

This is not a GMOA. Golden RiceB. TracyC. Bt BrinjalD. Dolly

Answer» Correct Answer - D
123.

Gene encoding Bt protein, specific for cotton bollworm isA. cry II AcB. cry II AbC. cry IAcD. cry II Abc

Answer» Correct Answer - C
124.

Which is not a pharmaceutical produce obtained through biotechnologyA. Human insulinB. Clotting factorC. CholecystokininD. Human growth hormone

Answer» Correct Answer - C
125.

Product of biotechnology isA. Transgenic cropB. BiofertilizerC. HumulinD. All the above

Answer» Correct Answer - D
126.

In plant biotechnology, root tumours are induced byA. RhizobiumB. Agrobacterium tumefaciensC. Agrobacterium rhizogenesD. Agrobacterium basilis

Answer» Correct Answer - C
127.

The plasmid used by Cohen and Boyer for the first transformation experiment wasA. pSC 101B. PUC 19C. pBR 322D. pBR 400

Answer» Correct Answer - A
128.

Modern biotechnology is based onA. Protoplast fusionB. Tissue cultureC. Recombinant proteinsD. Genetic engineering

Answer» Correct Answer - D
129.

Which of the following has revolutionised the discipline of biotechnologyA. Restriction endonucleasesB. Discovery of DNA structureC. Recombinant DNAD. All the these

Answer» Correct Answer - D
130.

The genetically modified crops (GM) may contain the gene of interest fromA. Closely related speciesB. Distantly related speciesC. Bacteria or animalsD. All the above

Answer» Correct Answer - D
131.

Study the lists and find the correct match A. a-iii, b-iv, c-ii, d-vB. a-iv, b-iii, c-i, d-vC. a-ii, b-iii, c-v, d-ivD. a-v, b-i, c-iii, d-ii

Answer» Correct Answer - A
132.

EcoRI is..........A. A restriction enzymeB. A plasmidC. Used to join two DNA fragmentsD. The abbreviation for bacterium Escherichia coli.

Answer» Correct Answer - A
133.

Identify the desirable characteristics for a plasmid used in rDNA technology from the following (a) Ability to multiply and express outside the host in a bioreactor (b) A highly active promoter (c) A site at which replication can be initiated (d) One or more identifiable marker genes (e) One or more unique restriction sitesA. a, c and e onlyB. b, c and e onlyC. a, c,d and e onlyD. b,c,d and e only

Answer» Correct Answer - D
134.

In an experiment the gene of bioluminescence from firefly has been successfully transferred into a plant as a result of which it started to glow. In the terminology of the molecular genetics, the plant is calledA. Firefly plantB. Hybrid plantC. Transgenic plantD. Bioluminescent plant

Answer» Correct Answer - C
135.

What is the advantage of clinical use of humulin over use of conventional ox of pig insulinA. It does not cause immunological problemsB. It is cheaper for the patientC. It is produced by E. coli in our intestineD. There is no advantage

Answer» Correct Answer - A
136.

Identify a,b,c,d in the given diagram A. a-ori, b-`amp^(R)`, c- `tet^(R)`, d- Hind IIIB. a- Hind III, b - `tet^(R)`, c - `amp^(R)`, d-oriC. a - `amp^(R)`, b - `tet^(R)`, c - Hind III, d - oriD. a - `tet^(R)`, b - Hind III, c - ori, d - `amp^(R)`

Answer» Correct Answer - A
137.

One of the methods of which DNA cannot be transferred to the host cell is byA. MicroinjectionB. Gene gunC. Disarmed pathogen vectorsD. Disarmed pathogen vectors

Answer» Correct Answer - D
138.

Golden Rice will help inA. Producing petrol-like fuelB. Pest resistanceC. Herbicide toleranceD. Alleviation of vitamin A deficiency

Answer» Correct Answer - D
139.

Which one of these is not a tool of recombinant DNA technologyA. Restriction enzymeB. VectorC. Polymerase enzymeD. Introns

Answer» Correct Answer - D
140.

Electroporation isA. Making transient pores in cell membranes to introduce gene constructsB. Fast passage of nutrients through phloem sieve pores by electric stimulationC. Opening of stomata by artificial light during nightD. Purification of saline water with the help of membrane system

Answer» Correct Answer - A
141.

Assertion. Agrobacterium tumefaciene is popular in genetic engineering because this bacterium is associated with roots of all cereals and pulse crops Reason. A gene incorporated in the bacterial chromosomal genome gets automatically transferred to the crop with which the bacterium is associated.A. both are true with reason being correct explanationB. both true but reason is not correct explanationC. assertion is true but reason is wrongD. both are wrong

Answer» Correct Answer - D
142.

Enzyme used in recombinant DNA technology (RDT) isA. LigasesB. PolymeraseC. Restriction endonucleaseD. Helicases

Answer» Correct Answer - C
143.

Assertion. In recombinant DNA technology, human genes are often transferred into bacteria or yeast. Reason. Both bacteria and yeast multiply very fast to form huge population which expresses the desired geneA. both are true with reason being correct explanationB. both true but reason is not correct explanationC. assertion is true but reason is wrongD. both are wrong

Answer» Correct Answer - A
144.

Assertion : In recombinant DNA technology, human genes are often transferred into bacteria or yeasts. Reason : Both bacteria and yeasts multiply very fast to form huge populations which express the desired genesA. if both are true with reason being correct explanationB. assertion true but correct explanationC. assertion true but reason is wrongD. both are wrong

Answer» Correct Answer - A
145.

What are correctly matched : 1. Ligase - Joins short segments of DNA together 2. DNA polymerase - cuts DNA at specific site 3. Helicase - breaks hydrogen bond between complementary pairs during DNA replicationA. 1,2,3 correctB. 1,2 correctC. 2,3 correctD. 1, 3 correct

Answer» Correct Answer - D
146.

DNA polymerase is isolated from bacteriaA. E. coliB. Bacillus thuringiensisC. Thermus aquaticusD. Agrobacterium

Answer» Correct Answer - C
147.

A technique which involves deliberate manipulation of genes within or between species is called:A. Gene therapyB. Tissue cultureC. Hybridoma technologyD. Genetic engineering

Answer» Correct Answer - D
148.

Cry protoxin is changed into toxic state inA. Alkaline mediumB. Acidic mediumC. Neutral mediumD. Contact with saliva

Answer» Correct Answer - A
149.

Amplification of gene of interest by using DNA polymerase may go uptoA. 0.1 millionB. 1.0 millionC. 1.0 billionD. 1.0 trollion

Answer» Correct Answer - C
150.

Gene amplification using primers can be done by :A. MicroinjectionB. ELISHAC. Polymerase chain reactionD. Gene gun

Answer» Correct Answer - C