82 + Interview Questions in Principles of Bioenergetics and Glycolysis in Biochemistry Page 2 InterviewSolution

51.	Which of the following enzyme catalyzes the first step in the catabolism of ethanol?(a) Alcohol dehydrogenase(b) Lactate dehydrogenase(c) Glutamate dehydrogenase(d) Malate dehydrogenaseThis question was addressed to me in unit test.Question is taken from Biological Oxidation-Reduction Reactions topic in chapter Principles of Bioenergetics and Glycolysis of Biochemistry
Answer» The correct OPTION is (a) Alcohol dehydrogenase Best EXPLANATION: Alcohol dehydrogenase CATALYZES the FIRST step in the CATABOLISM of ethanol.

Discussion

52.	For a reaction if ∆G° is positive, then ____________(a) The products will be favored(b) The reactants will be favored(c) The concentration of the reactants and products will be equal(d) All of the reactant will be converted to productsThe question was asked by my school teacher while I was bunking the class.My doubt stems from Bioenergetics and Thermodynamics in section Principles of Bioenergetics and Glycolysis of Biochemistry
Answer» The correct OPTION is (B) The reactants will be FAVORED To explain I would SAY: If ∆G° is NEGATIVE, products are favored.

Discussion

53.	What is the main source of glucose carbons for gluconeogenesis?(a) Guanine(b) Alanine(c) Cysteine(d) ThreonineThis question was posed to me in an internship interview.The doubt is from Gluconeogenesis topic in section Principles of Bioenergetics and Glycolysis of Biochemistry
Answer» Right option is (b) Alanine The BEST I can explain: The main source of GLUCOSE carbons for GLUCONEOGENESIS is alanine DERIVED from the breakdown of muscle proteins.

Discussion

54.	Gluconeogenesis involves the conversion of ____________(a) Glucose to pyruvate(b) Pyruvate to glucose(c) Phosphoenolpyruvate to glucose(d) Pyruvate to fructoseI got this question in an interview.The origin of the question is Gluconeogenesis in chapter Principles of Bioenergetics and Glycolysis of Biochemistry
Answer» The CORRECT option is (b) Pyruvate to GLUCOSE Explanation: Gluconeogenesis is the PROCESS of CONVERSION of pyruvate to glucose whereas glycolysis is the process of conversion of glucose to pyruvate.

Discussion

55.	TPP (thiamine pyrophosphate) is derived from ____________(a) Vitamin A(b) Vitamin B1(c) Vitamin C(d) Vitamin B2The question was posed to me in homework.My doubt stems from Fates of Pyruvate under Anaerobic Conditions: Fermentation topic in portion Principles of Bioenergetics and Glycolysis of Biochemistry
Answer» Correct CHOICE is (b) Vitamin B1 The best I can EXPLAIN: TPP is a coenzyme DERIVED from vitamin B1 (THIAMINE).

Discussion

56.	What is the general term used for the anaerobic degradation of glucose to obtain energy?(a) Anabolism(b) Oxidation(c) Fermentation(d) MetabolismThis question was posed to me in an interview.This interesting question is from Glycolysis topic in chapter Principles of Bioenergetics and Glycolysis of Biochemistry
Answer» The correct CHOICE is (c) Fermentation To explain I would say: Fermentation is the GENERAL term USED for the degradation of glucose to obtain energy in the form of ATP.

Discussion

57.	Which out of the following enzymes can utilize both NAD^+ and NADP^+ as a coenzyme?(a) Glutamate dehydrogenase(b) Alcohol dehydrogenase(c) Aldehyde dehydrogenase(d) Glycerol-3-P dehydrogenaseThe question was posed to me during an interview.Asked question is from Biological Oxidation-Reduction Reactions in chapter Principles of Bioenergetics and Glycolysis of Biochemistry
Answer» The CORRECT OPTION is (a) Glutamate dehydrogenase Explanation: Only Glutamate dehydrogenase can UTILIZE both NAD^+ and NADP^+ as a coenzyme.

Discussion

58.	Which of the following statements is incorrect about oxidase?(a) They catalyze reactions involving hydrogen peroxide(b) They catalyze reaction using O2 as a hydrogen acceptor(c) They catalyze reactions using niacin as a coenzyme(d) They catalyze reactions of direct incorporation of O2 into the substrateThis question was addressed to me in final exam.Query is from Biological Oxidation-Reduction Reactions topic in section Principles of Bioenergetics and Glycolysis of Biochemistry
Answer» The correct choice is (B) They CATALYZE reaction using O2 as a HYDROGEN acceptor To explain: They catalyze REACTIONS involving hydrogen peroxide, reactions using niacin as COENZYME and reactions of direct incorporation of O2 into the substrate.

Discussion

59.	Which of the following statements about redox potential is false?(a) NADH/NAD^+ redox pair has the least redox potential(b) Oxygen/H2O redox pair has the highest redox potential(c) The components of the electron transport chain are organized in terms of their redox potential(d) The redox potential of a system is usually compared with the potential of the hydrogen electrodeI have been asked this question in my homework.I would like to ask this question from Biological Oxidation-Reduction Reactions topic in portion Principles of Bioenergetics and Glycolysis of Biochemistry
Answer» The CORRECT answer is (a) NADH/NAD^+ redox PAIR has the least redox POTENTIAL To explain: NAD^+/NADH redox pair has the least redox potential.

Discussion

60.	Which is an example of chemical to osmotic energy conversion that occurs in living organisms?(a) ATP-driven muscle contraction(b) ATP-dependent photon emission in fireflies(c) light-induced electron flow in chloroplasts(d) ATP-driven active transport across a membraneThe question was posed to me by my college professor while I was bunking the class.I would like to ask this question from Phosphoryl Group Transfers and ATP topic in section Principles of Bioenergetics and Glycolysis of Biochemistry
Answer» The correct option is (d) ATP-driven ACTIVE transport across a membrane The best explanation: ATP-driven muscle contraction is an EXAMPLE for chemical to mechanical energy CONVERSION. ATP-dependent photon emission in fireflies is an example for chemical to electromagnetic energy conversion. Light-induced ELECTRON FLOW in chloroplasts is an example for electromagnetic to electrical energy conversion.

Discussion

61.	Which of the following statements is false?(a) The reaction tends to go in the forward direction if ∆G is large and positive(b) The reaction tends to move in the backward direction if ∆G is large and negative(c) The system is at equilibrium if ∆G = 0(d) The reaction tends to move in the backward direction if ∆G is large and positiveI have been asked this question during an online exam.The above asked question is from Bioenergetics and Thermodynamics topic in division Principles of Bioenergetics and Glycolysis of Biochemistry
Answer» RIGHT choice is (d) The reaction tends to move in the BACKWARD direction if ∆G is large and positive For explanation I WOULD say: The reaction tends to move in the backward direction if ∆G is large and negative.

Discussion

62.	Which one out of the following enzymes acts in the pentose phosphate pathway?(a) Aldolase(b) Glycogen phosphorylase(c) Pyruvate kinase(d) 6-phosphogluconate dehydrogenaseThis question was posed to me in exam.My question is from Pentose Phosphate Pathway of Glucose Oxidation in chapter Principles of Bioenergetics and Glycolysis of Biochemistry
Answer» The CORRECT answer is (d) 6-phosphogluconate dehydrogenase The explanation is: The FIRST reaction in PENTOSE phosphate pathway is CATALYZED by 6-phosphogluconate dehydrogenase.

Discussion

63.	In the pentose phosphate pathway, the major products are ____________(a) Ribulose and NADPH(b) Ribulose and NADH(c) Ribulose and NAD^+(d) Ribulose and ATPThe question was asked in a job interview.This intriguing question comes from Pentose Phosphate Pathway of Glucose Oxidation topic in division Principles of Bioenergetics and Glycolysis of Biochemistry
Answer» The correct answer is (a) Ribulose and NADPH To explain I WOULD say: The PRODUCTS formed are PENTOSE, ribulose and NADPH.

Discussion

64.	Oxaloacetate is reduced to malate by ____________(a) Pyruvate carboxylase(b) Malate dehydrogenase(c) Pyruvate kinase(d) Phosphofructokinase-1This question was posed to me in my homework.Query is from Gluconeogenesis topic in portion Principles of Bioenergetics and Glycolysis of Biochemistry
Answer» Correct option is (b) Malate DEHYDROGENASE To EXPLAIN: Oxaloacetate FORMED from PYRUVATE is REDUCED to malate by malate dehydrogenase.

Discussion

65.	Which of the following enzyme is present in saliva?(a) Hexokinase(b) Alpha amylase(c) Fructokinase(d) Triose kinaseI have been asked this question in exam.I'd like to ask this question from Feeder Pathways for Glycolysis topic in chapter Principles of Bioenergetics and Glycolysis of Biochemistry
Answer» Right answer is (b) ALPHA AMYLASE Best explanation: DIGESTION begins in the mouth, where SALIVARY alpha amylase hydrolyses starch to sucrose.

Discussion

66.	High concentration of glucose 6-phosphate is inhibitory to ___________(a) Hexokinase(b) Pyruvate kinase(c) Glucokinase(d) Phosphofructokinase-1The question was posed to me in examination.This intriguing question comes from Glycolysis topic in chapter Principles of Bioenergetics and Glycolysis of Biochemistry
Answer» RIGHT choice is (a) HEXOKINASE The best explanation: Hexokinase catalyzes the reaction involving conversion of GLUCOSE to glucose 6-phosphate.

Discussion

67.	If ∆G^‘° of the reaction A → B is -40kJ/mol under standard conditions then the reaction ____________(a) Will never reach equilibrium(b) Will not occur spontaneously(c) Will proceed at a rapid rate(d) Will proceed from left to right spontaneouslyThis question was addressed to me in an interview for internship.My question comes from Bioenergetics and Thermodynamics topic in division Principles of Bioenergetics and Glycolysis of Biochemistry
Answer» The correct choice is (d) Will proceed from LEFT to right spontaneously To EXPLAIN I would say: If ∆G^‘° < 0, REACTION PROCEEDS from left to right that means products are favored.

Discussion

68.	When one molecule of glucose is oxidized to two molecules of lactate during anaerobic glycolysis, which of the following statements is false?(a) Glyceraldehyde 3-P dehydrogenase reaction produces 2 ATP molecules(b) Lactate dehydrogenase reaction produces no ATP(c) Pyruvate kinase reaction produces 2 ATP molecules(d) Phosphofructokinase-1 reaction uses 1 ATP moleculeI got this question in an interview for internship.My question is based upon Fates of Pyruvate under Anaerobic Conditions: Fermentation topic in section Principles of Bioenergetics and Glycolysis of Biochemistry
Answer» Correct choice is (a) GLYCERALDEHYDE 3-P dehydrogenase REACTION produces 2 ATP molecules To explain: When one molecule of glucose is oxidized to two molecules of lactate during ANAEROBIC glycolysis, Glyceraldehyde 3-P dehydrogenase reaction does not produce 2 ATP molecules.

Discussion

69.	For its activity, pyruvate decarboxylase requires ______________(a) Mg^+2(b) Ca^+2(c) Na^+(d) H^+I had been asked this question in a job interview.I'm obligated to ask this question of Fates of Pyruvate under Anaerobic Conditions: Fermentation in chapter Principles of Bioenergetics and Glycolysis of Biochemistry
Answer» Correct OPTION is (a) Mg^+2 The EXPLANATION: Pyruvate decarboxylase requires Mg^+2 in the conversion of pyruvate to ACETALDEHYDE.

Discussion

70.	What is the first step in the payoff phase of glycolysis?(a) Reduction of 1, 3-bisphosphoglycerate to glyceraldehyde 3-phosphate(b) Oxidation of glyceraldehyde 3-phosphate to 1, 3-bisphosphoglycerate(c) Reversible conversion of dihydroxyacetone phosphate to glyceraldehyde 3-phosphate(d) Irreversible conversion of dihydroxyacetone phosphate to glyceraldehyde 3-phosphateI had been asked this question in an online quiz.My question is based upon Glycolysis in section Principles of Bioenergetics and Glycolysis of Biochemistry
Answer» Right choice is (b) OXIDATION of glyceraldehyde 3-phosphate to 1, 3-bisphosphoglycerate The explanation is: Glyceraldehyde 3-phosphate dehydrogenase catalyzes the FIRST step in the PAYOFF phase, oxidation of glyceraldehyde 3-phosphate to 1, 3-bisphosphoglycerate.

Discussion

71.	The relationship between K^‘eq and ∆G^‘° is?(a) ∆G^‘° = RTlnK^‘eq(b) ∆G^‘° = -RTK^‘eq(c) ∆G^‘° = RK^‘eq(d) ∆G^‘° = -RK^‘eqI had been asked this question at a job interview.Query is from Bioenergetics and Thermodynamics in portion Principles of Bioenergetics and Glycolysis of Biochemistry
Answer» RIGHT choice is (b) ∆G^‘° = -RTK^‘eq Easy EXPLANATION: If the system is in EQUILIBRIUM ∆G° = 0 and ∆G^‘° = -RTK^‘eq is the correct RELATION between K^‘eq and ∆G^‘°.

Discussion

72.	If enthalpy change for a reaction is zero, then ∆G° equals to _____________(a) -T∆S°(b) T∆S°(c) -∆H°(d) lnkeqI had been asked this question in an interview for internship.Question is taken from Bioenergetics and Thermodynamics in portion Principles of Bioenergetics and Glycolysis of Biochemistry
Answer» The CORRECT ANSWER is (a) -T∆S° Easy EXPLANATION: From the EQUATION, ∆G° = ∆H°-T∆S° If∆H° = 0 then ∆G° = -T∆S°.

Discussion

73.	Which one out of the following is not a NAD^+ requiring enzyme?(a) Lactate dehydrogenase(b) Pyruvate dehydrogenase complex(c) Maltate dehydrogenase(d) Acyl co-A dehydrogenaseI have been asked this question during an online interview.My query is from Biological Oxidation-Reduction Reactions in section Principles of Bioenergetics and Glycolysis of Biochemistry
Answer» Right OPTION is (d) Acyl co-A DEHYDROGENASE To EXPLAIN: Acyl co-A dehydrogenase is not a NAD^+ requiring enzyme.

Discussion

74.	Which of the following enzyme is responsible for the conversion of glucose 1-phosphate to glucose 6-phosphate?(a) Glycogen phosphorylase(b) Isomerase(c) Phosphoglucomutase(d) EpimeraseThis question was posed to me in a national level competition.Query is from Feeder Pathways for Glycolysis in portion Principles of Bioenergetics and Glycolysis of Biochemistry
Answer» The CORRECT OPTION is (c) Phosphoglucomutase To EXPLAIN I WOULD say: Phosphoglucomutase converts the GLUCOSE 1-phosphate to glucose 6-phosphate, which can enter glycolysis.

Discussion

75.	Which of the following enzyme catalyzes the first step of glycolysis?(a) Hexokinase(b) Pyruvate kinase(c) Glucokinase(d) Phosphofructokinase-1I have been asked this question by my college professor while I was bunking the class.The origin of the question is Glycolysis in division Principles of Bioenergetics and Glycolysis of Biochemistry
Answer» The correct answer is (a) Hexokinase Explanation: The FIRST of GLYCOLYSIS is IRREVERSIBLE under intracellular CONDITIONS and is CATALYZED by hexokinase.

Discussion

76.	What is the relationship between ∆G and ∆G^‘°?(a) ∆G = ∆G^‘° + RTln([products]/[reactants])(b) ∆G = ∆G^‘° – RTln([products]/[reactants])(c) ∆G = ∆G^‘° + RTln([reactants]/[products])(d) ∆G = ∆G^‘° – RTln([reactants]/[products])This question was posed to me during an online interview.The above asked question is from Bioenergetics and Thermodynamics topic in division Principles of Bioenergetics and Glycolysis of Biochemistry
Answer» The correct ANSWER is (a) ∆G = ∆G^‘° + RTln([PRODUCTS]/[reactants]) The best EXPLANATION: ∆G = ∆G^‘° + RTln([products]/[reactants] ) is the correct RELATION between ∆G and ∆G^‘°.

Discussion

77.	What is the main function of the pentose phosphate pathway?(a) Supply NADP^+(b) Supply NADH(c) Supply energy(d) Supply pentoses and NADPHThe question was asked during an interview.Question is taken from Pentose Phosphate Pathway of Glucose Oxidation topic in section Principles of Bioenergetics and Glycolysis of Biochemistry
Answer» The CORRECT choice is (d) Supply pentoses and NADPH Explanation: The main FUNCTION of pentose phosphate PATHWAY is to supply pentoses to NADPH.

Discussion

78.	Which one of the following statements is correct about the pentose phosphate pathway?(a) It generates 6 moles of CO2 for each mole of glucose consumed(b) It generates 38 mol of ATP per mole of glucose consumed(c) It is a reductive pathway that consumes NADH(d) Present in plants but not in animalsThis question was posed to me in quiz.The question is from Pentose Phosphate Pathway of Glucose Oxidation topic in division Principles of Bioenergetics and Glycolysis of Biochemistry
Answer» RIGHT option is (a) It generates 6 moles of CO2 for each MOLE of glucose consumed The best I can explain: There is the continued PRODUCTION of NADPH and CONVERTING glucose 6-phosphate (in six cycles) to CO2.

Discussion

79.	Hydrolysis of lactose yields ___________(a) D-galactose and D-glucose(b) D-glucose and D-glucose(c) D-galactose and D-fructose(d) D-fructose and D-glucoseThis question was addressed to me in an online interview.Query is from Feeder Pathways for Glycolysis in chapter Principles of Bioenergetics and Glycolysis of Biochemistry
Answer» The CORRECT CHOICE is (a) D-galactose and D-glucose The best I can EXPLAIN: Hydrolysis of lactose yields D-galactose and D-glucose.

Discussion

80.	Glycolysis converts ___________(a) Glucose into pyruvate(b) Glucose into phosphoenolpyruvate(c) Fructose into pyruvate(d) Fructose into phosphoenolpyruvateI had been asked this question in homework.Asked question is from Glycolysis in division Principles of Bioenergetics and Glycolysis of Biochemistry
Answer» Right answer is (a) GLUCOSE into pyruvate The explanation: GLYCOLYSIS is a METABOLIC PATHWAY that converts glucose into pyruvate.

Discussion

81.	The product formed in the first substrate level phosphorylation in glycolysis is ___________(a) Pyruvate(b) 3-phosphoglycerate(c) 1, 3-bisphosphoglycerate(d) 2-phosphoglycerateThe question was posed to me during an online interview.I would like to ask this question from Glycolysis in section Principles of Bioenergetics and Glycolysis of Biochemistry
Answer» The correct OPTION is (b) 3-PHOSPHOGLYCERATE Easy explanation: Phosphoglycerate kinase TRANSFERS PHOSPHORYL group from 1, 3-bisphosphoglycerate to ADP forming ATP and 3-phosphoglycerate.

Discussion

82.	Which is an example of chemical to mechanical energy conversion that occurs in living organisms?(a) ATP-driven muscle contraction(b) ATP-dependent photon emission in fireflies(c) light-induced electron flow in chloroplasts(d) ATP-driven active transport across a membraneThe question was posed to me in semester exam.My doubt is from Phosphoryl Group Transfers and ATP in portion Principles of Bioenergetics and Glycolysis of Biochemistry
Answer» RIGHT CHOICE is (a) ATP-driven muscle contraction The BEST I can EXPLAIN: ATP-dependent photon EMISSION in fireflies is an example for chemical to electromagnetic energy conversion. Light-induced electron flow in chloroplasts is an example for electromagnetic to electrical energy conversion. ATP-driven active transport across a membrane is an example for chemical to osmotic energy conversion.

Discussion

Explore topic-wise InterviewSolutions in .

What is the main source of glucose carbons for gluconeogenesis?(a) Guanine(b) Alanine(c) Cysteine(d) ThreonineThis question was posed to me in an internship interview.The doubt is from Gluconeogenesis topic in section Principles of Bioenergetics and Glycolysis of Biochemistry

Oxaloacetate is reduced to malate by ____________(a) Pyruvate carboxylase(b) Malate dehydrogenase(c) Pyruvate kinase(d) Phosphofructokinase-1This question was posed to me in my homework.Query is from Gluconeogenesis topic in portion Principles of Bioenergetics and Glycolysis of Biochemistry

Which of the following enzyme is present in saliva?(a) Hexokinase(b) Alpha amylase(c) Fructokinase(d) Triose kinaseI have been asked this question in exam.I'd like to ask this question from Feeder Pathways for Glycolysis topic in chapter Principles of Bioenergetics and Glycolysis of Biochemistry