1.

A solution prepared by dissolving 8.95 mg of a gene fragment in35.0 ml of water has an osmotic pressure of 0.335 ton at 25^(@)C. Assuming thegene fragment is a non-electrolyse, determine the molar mass.

Answer»

Solution :Mass of gene fragment = 8.95 mg
= `8.95 xx 10^(-3) g`
Volume of water = `35.0 ML = 35 xx 10^(-3)` L
`pi = 0.335` ton = 0.335/760 atm
Temp = 25 + 273 = 298 K
`pi = (W_(B) RT)/(M_(B) xx V)`
`(0.335)/(760) = (8.95 xx 10^(-3) xx 0.0821 xx 298)/(M_(B) xx 35 xx 10^(-3))`
`M_(B) = 141933 g MOL^(-3)`


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