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Explain aboutDNAfingerprintingprocess. |
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Answer» Solution :(i) DNA fingerprinting is oneof the mostaccuratemethods for placingan individualat thescene ofa crimehas beena FINGER print. (ii)The DNAfingerprintisuniquefor everyperson and can be extractedformtracresof sample formblood,SALIVA,hairetc. By using thismethod, we candetect the individual specificvariationin human DNA. (iii) In thismethod ,theextracted DNAIS cutat specificpointsof varying lenghthsin theformationof DNAfragmentsof varying lengths whichwereanalysedby techniquecalledgel electrophoresis. Thismethodsepratesthe fragmentsbasedon theirsize. Thegel containingthe DNAfragmentsare thentransferredto a nylon sheetusinga techniquecalledblotting . Then the fragmentswillundergoautoradiography in WHICHTHEY wereexposed to DNAprobes. (iv)A piece of X -rayfilm was then exposed tothefragments , a anda darkmark was producedat any pointwhere a radioactivewithothersamples. (v) DNAfingerprintingis BASEDON slightsequencedifferencebetweenindividuals . These methods are providingdeceisivein courtcases world wide . |
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