From a mixture of amino acids the constituents can be separated by

1.	From a mixture of amino acids the constituents can be separated by
Answer» ion-exchange chromatography electro-osmosis electrophoresis Both (1) and (3) Solution :Electrophoresis can be used to separate and identify amino acids: If a filter paper-strip moistenedwith a solution of a mixture of `A A's` is placed between two electrodes, the charged MOLECULE will migrate to one electrode or the other at a RATE that depends on its net charge and the applied voltage. The net charge depend on the pH. The strip is then STAINED with a reagent that reacts with the `A A`, there by forming a colour whose position on the strip is COMPARED for identification with that of a known sample. A mixture of `A A's` can also be separated by ion-exchange chromatoography: the column is charged with an ion-exchange resin with charged groups at tis surface. A cation - exchange resin, i.e., `R - SO_(3)^(-) NA^(+) (R=` resin), exchanges it cation`(Na^(+))` for positively charged `A A's` in acid when most `A A's` are cations. Separation depends on the rate of movement of the positively charged `A A's` down the column as the exchange occurs. The rate of movement is inversely proportional to the magnitude to the + charge on the `A A`.

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