Prolonged use, excessive alkaline immersion, or high-temperature operation will cause surface leaching of the membrane glass. The RESULT is extremely noisy and/or sluggish response, which cannot be remedied simply by cleaning the electrode. If this OCCURS, the following procedures will often provide stability and pH sensitivity. Always consider the electrode’s materials of construction before using these procedures.

1. Empty the reference chamber, rinse with deionised water, empty and refill with the specified filling solution.
2. Soak the electrode in hot (50°C – 60°C) reference electrolyte for a few minutes.
3. Soak the electrode overnight in pH 4 buffer.
4. Remove any exterior salt deposits with DISTILLED water.
5. If the filling solution does not flow through the junction by this time (generally due to an unusually low junction porosity), use gentle suction to pull filling solution through the junction and repeat from step 2.
6. Sometimes the material clogging the junction requires more SEVERE action. Should the above fail, proceed as follows:

• Use a solvent specific to the solution or material plugging the junction, if possible.
• Soak the membrane overnight in 0.1 M HCI.
• If measurements have been made in samples containing protein, remove protein deposits by soaking the electrode bulb in 0.1 M HCl containing 1% pepsin.
• Repeat from step 1.

If all these fail, the electrode should be discarded safely and replaced.

Prolonged use, excessive alkaline immersion, or high-temperature operation will cause surface leaching of the membrane glass. The result is extremely noisy and/or sluggish response, which cannot be remedied simply by cleaning the electrode. If this occurs, the following procedures will often provide stability and pH sensitivity. Always consider the electrode’s materials of construction before using these procedures.

If all these fail, the electrode should be discarded safely and replaced.