In a paper electrophoresis amino acids and peptides can be separated by their differential migration in an electric field. To the center of a strip of paper, wet with buffer at pH=6 is applied a mixture of the following three peptides in a single small spot: Gly-Ala, Gly-Asp and Gly-Arg. A positively charged electrode (anode) is attached to the left side of the paper and a negatively charged electrode (cathode) to the rightside. A voltage is applied across the ends of the paper for a time, after which the peptides have separated into three spots. One near the location of the original spot whtch peptide is in each spot? Explain.

In a paper electrophoresis amino acids and peptides can be separated b

1.	In a paper electrophoresis amino acids and peptides can be separated by their differential migration in an electric field. To the center of a strip of paper, wet with buffer at pH=6 is applied a mixture of the following three peptides in a single small spot: Gly-Ala, Gly-Asp and Gly-Arg. A positively charged electrode (anode) is attached to the left side of the paper and a negatively charged electrode (cathode) to the rightside. A voltage is applied across the ends of the paper for a time, after which the peptides have separated into three spots. One near the location of the original spot whtch peptide is in each spot? Explain.
Answer» SOLUTION :Gly-Asp will MOVE towards ANODE while Gly-Arg will move towards calhode and Gly-Ald will remain HEAR SPOT.