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In which of this method electric field is applied for gene transfer?(a) Electroporation(b) Microinjection(c) Particle bombardment(d) SonoporationI had been asked this question in an online quiz.I want to ask this question from Genetic Recombination in chapter Genetic Recombination of Drug Biotechnology

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Correct choice is (a) Electroporation

The best explanation: In electroporation ELECTRIC field is applied ACROSS the cells suspended in an electroporation cuvette. The high voltage for a few milliseconds is ESSENTIAL. Since this will create transient pores on the cell membrane which will get REFILLED again.



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