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Insulin (C_(2)H_(10)O_(5))_(n) is dissolved in a suitable solvent and the osmotic pressure (pi) of solutions of various concentrations (C) is measured at 20^(@)C. The slope of the plot of pi (atm) versus C (in g//cm^(3)) is found to be 4.65xx10^(-3). The molecular weight of insulin is |
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Answer» `3.17xx10^(6)` Thus, C' in the formula should be replaced by this factor, i.e., `pi=(Cxx1000)/(M_(2))RT=(1000RT)/(M_(2))C` Thus, a plot of `pi` VS C will be linear with SLOPE `=(1000RT)/(M_(2))=4.65xx10^(-3)"(Given)"` `therefore (1000xx0.0821xx293)/(M_(2))=4.65xx10^(-3)` `"or"M_(2)=5.17xx10^(6)` |
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