Question : Let us tak a smallpiece from an onion bulb.With the help of a pair of forceps. We can peel off the skin (called epidermis) from the concave side (inner layer) of the onion. This layer can be put immediately in a watch-glass containing water. This will prevent the peel from getting folded or getting dry. Let us take a glass slide, put a drop of water on it and transfer a small piece of the peel from the watch-glass to the slide. Make sure that the peel is perfectly flat on the slide. A thin camel hair paintbrush might be necessary to help transfer the peel. Now we put a drop of safranin solution on this piece followed by a cover slip. Take care to avoid air bubbles while putting the cover slip with the help of a mounting needle. We have prepared a temporary mount of onion peel. We can observe this slide under low power followed by high powers of a compound microscope. What do we observe as we look through the lens ? What do we observe as we look through the lens ?

Question : Let us tak a smallpiece from an onion bulb.With the help of

1.	Question : Let us tak a smallpiece from an onion bulb.With the help of a pair of forceps. We can peel off the skin (called epidermis) from the concave side (inner layer) of the onion. This layer can be put immediately in a watch-glass containing water. This will prevent the peel from getting folded or getting dry. Let us take a glass slide, put a drop of water on it and transfer a small piece of the peel from the watch-glass to the slide. Make sure that the peel is perfectly flat on the slide. A thin camel hair paintbrush might be necessary to help transfer the peel. Now we put a drop of safranin solution on this piece followed by a cover slip. Take care to avoid air bubbles while putting the cover slip with the help of a mounting needle. We have prepared a temporary mount of onion peel. We can observe this slide under low power followed by high powers of a compound microscope. What do we observe as we look through the lens ? What do we observe as we look through the lens ?
Answer» Solution : If the epidermal fold of the onion bulb ismounted on the slide and observed we can observe following structures in this temporary mounting: (1) Epidermal cells which are slightly RECTANGULAR in shape. (2) All the cells are SIMILAR in shape and size. (3) There is nucleus which takes up the stain. (4) Cytoplasm surrounds the nucleus. (5) In high power, the nucleus is clearly seen and a faint outline of cell wall is also visible.