Variation in pH of medium result in changes of ionic form of the active site.(a) True(b) FalseThis question was posed to me in class test.My doubt is from Methods of Immobilization topic in chapter Antibiotics & Enzyme Immobilization of Drug Biotechnology

Variation in pH of medium result in changes of ionic form of the activ

1.	Variation in pH of medium result in changes of ionic form of the active site.(a) True(b) FalseThis question was posed to me in class test.My doubt is from Methods of Immobilization topic in chapter Antibiotics & Enzyme Immobilization of Drug Biotechnology
Answer» The correct answer is (a) True Easy explanation: Variation in pH of MEDIUM result in changes of IONIC form of the active site and activity of enzyme, hence the reaction rate. Variation in pH of medium can also result in changes of the maximum reaction rate, Km stability of the enzyme. Enzyme are active only over small RANGE of pH due to the active site functional group charges (ionic form) and the three dimensional SHAPE of enzyme are pH-dependent.

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