What do you add to Myeloma cells to receive HGPRT- myeloma cells?(a) 8

1.	What do you add to Myeloma cells to receive HGPRT- myeloma cells?(a) 8 – Azaguanine(b) Azaguanine(c) Nitrogen flush(d) Carbon dioxide flushI got this question in final exam.I'd like to ask this question from Genetic Recombination topic in section Genetic Recombination of Drug Biotechnology
Answer» Right OPTION is (a) 8 – Azaguanine Best explanation: The myeloma cells harvested from the antigen injected mouse has to be added with 8-Azaguanine. 8-Azaguanine tends to stop the de NOVO PATHWAY of and the cells which cannot survive will DIE. Myeloma cells which are HGPRT- will be the only cells growing in the culture due to the presence of the compound. These cells will have high viability and rapid growth.

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What do you add to Myeloma cells to receive HGPRT- myeloma cells?(a) 8 – Azaguanine(b) Azaguanine(c) Nitrogen flush(d) Carbon dioxide flushI got this question in final exam.I'd like to ask this question from Genetic Recombination topic in section Genetic Recombination of Drug Biotechnology

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