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What Is Acid-alcohol Decolourizer? |
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Answer» 3% HCI in 95% alcohol (methylated spirit). This is useful in dilrerentiating saprophytic Mycobacteria from pathogenic Mycobacteria Pathogenic Mycobacteria are both acid and alcohol fast but saprophytic Mycobacterla are only acid-fast Saprophytic Mycobacterla can get declourized by alcohol. 95% alcohol can be USED as a secondary decolorizer after decolourizing with acid Especially used in staining smears prepared from URINE that may CONTAIN Mycobacterium smegmatis. 3% HCI in 95% alcohol (methylated spirit). This is useful in dilrerentiating saprophytic Mycobacteria from pathogenic Mycobacteria Pathogenic Mycobacteria are both acid and alcohol fast but saprophytic Mycobacterla are only acid-fast Saprophytic Mycobacterla can get declourized by alcohol. 95% alcohol can be used as a secondary decolorizer after decolourizing with acid Especially used in staining smears prepared from urine that may contain Mycobacterium smegmatis. |
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