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InterviewSolution
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When are two objects just resolved ? Explain. How can the resolving power of a compound microscope be incresed ? Use relevant formula to support your answer. |
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Answer» Solution :Two objects are said to be just resolved when central diffraction MAXIMUM of one object coincides with first diffraction MINIMA of the other object and vice versa. Resolving power of a compound microscope `=(2nsina)/(1.22lamda)`, where n=refractive index of medium present between the object and objective LENS, 2D is the angle subtended by the objective lens at the object and `lamda` the wavelength of light used to ILLUMINATE the object. generally the term n sin `alpha` is known as numerical aperture (N.A.) of the microscope. thus, to increase the resolving power of a compound microscope (i) its numerical aperrture should be increased, and (ii) the wavelength of illuminating light should be reduced. |
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