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Which of the following are the characteristics of sonoporation?(a) Suspend the cells in an electroporation cuvette(b) Injecting the DNA into the cell(c) Also known as biolistics(d) Usage of ultrasoundThis question was addressed to me in unit test.The question is from Genetic Recombination topic in portion Genetic Recombination of Drug Biotechnology

Answer»

Right option is (d) Usage of ultrasound

To elaborate: Sonoporation USED ultrasound treatment for forming PORES on the cell membrane. A microbubble which has the DNA will be provided with s=ultra sound along with the cells in which DNA has to be INCORPORATED. The sound cause pores to be formed on the cell membrane and the bubble burst due to cavitation releasing the DNA into the SOLUTION.



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