Which of the following are the characteristics of bead transfection?(a) Suspend the cells in an electroporation cuvette(b) Physically breaking the cells(c) Also known as Biolistic(d) Usage of ultrasoundThe question was posed to me in final exam.I'd like to ask this question from Genetic Recombination in portion Genetic Recombination of Drug Biotechnology

Which of the following are the characteristics of bead transfection?(a

1.	Which of the following are the characteristics of bead transfection?(a) Suspend the cells in an electroporation cuvette(b) Physically breaking the cells(c) Also known as Biolistic(d) Usage of ultrasoundThe question was posed to me in final exam.I'd like to ask this question from Genetic Recombination in portion Genetic Recombination of Drug Biotechnology
Answer» Correct answer is (b) Physically breaking the cells The best explanation: Bead transfection COMBINES the principle of physically producing breaks in the cellular membrane using BEADS. In this method, the adherent cells are incubated for a brief period with glass beads in a solution containing the DNA. Suspending cells in electroporation cuvette is for electroporation. BIOLISTIC is ALSO KNOWN as particle bombardment. Ultrasound is used in sonoporation.

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