Which of the following do not incorporate in the genome and replicates in the cytoplasm?(a) Poxvirus(b) Baculovirus(c) Adenovirus(d) RetrovirusThis question was posed to me in an interview for internship.I'd like to ask this question from Genetic Recombination topic in section Genetic Recombination of Drug Biotechnology

Which of the following do not incorporate in the genome and replicates

1.	Which of the following do not incorporate in the genome and replicates in the cytoplasm?(a) Poxvirus(b) Baculovirus(c) Adenovirus(d) RetrovirusThis question was posed to me in an interview for internship.I'd like to ask this question from Genetic Recombination topic in section Genetic Recombination of Drug Biotechnology
Answer» Correct option is (a) Poxvirus Easy explanation: Poxvirus/ vaccinia virus does not get INCORPORATED into the genome of the host CELL. They reside in the cytoplasm and REPLICATE in the cytoplasm. Thus, they help in TRANSIENT transformation RATHER than permanent transformation. The genome size of pox/ vaccinia virus is 300 kb.

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