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101.

Transgenic plants carry desirable traits like _______(a) harmful genes(b) herbicide resistance(c) lactose intolerance(d) complementary genesI have been asked this question in a national level competition.I'd like to ask this question from Biotechnology Applications topic in portion Biotechnology and its Applications of Biology – Class 12

Answer»

The correct ANSWER is (b) herbicide resistance

The BEST I can explain: Transgenic plants carry desirable traits like herbicide resistance. Few HERBS are weeds that interfere with the growth of normal desirable plants. Transgenic plants also carry other CHARACTERISTICS like disease-resistance, insect-resistance, etc.

102.

Two bacteria found to be very useful in genetic engineering experiments are _______(a) Nitrosomonas and Klebsiella(b) Nitrobacter and Azotobacter(c) Escherichia and Agrobacterium(d) Rhizobium and DiplococcusThe question was posed to me in a national level competition.My doubt is from Biotechnology Applications in section Biotechnology and its Applications of Biology – Class 12

Answer»

Right ANSWER is (c) Escherichia and Agrobacterium

For explanation I would say: Escherichia and Agrobacterium are the two most widely used bacteria for genetic ENGINEERING. Escherichia is useful for obtaining MANY products such as insulin while Agrobacterium is used for producing BT crops.

103.

The transgenic plant ‘Flavr savr’ tomato carries an artificial gene for ______(a) delaying ripening process(b) added flavours(c) both delaying ripening process and added flavours(d) insect resistanceI got this question in a job interview.This interesting question is from Biotechnology Applications in division Biotechnology and its Applications of Biology – Class 12

Answer» RIGHT answer is (c) both DELAYING ripening process and added flavours

The best I can explain: The Flavr savr tomato carries an antisense PG gene which inhibits the activity of pectin degrading enzyme called polygalacturonase. This artificial gene delays the ripening process and increasing shelf LIFE. It also adds flavor to the tomato.
104.

The transgenic tomato variety with an improved shelf life is ______(a) Lyco 100(b) PHB tomato(c) Bt tomato(d) Flavr savrThe question was posed to me in an international level competition.Enquiry is from Biotechnology Applications in division Biotechnology and its Applications of Biology – Class 12

Answer» CORRECT answer is (d) Flavr savr

Best explanation: Flavr savr is a genetically modified tomato. It was the first GM food crop to be used commercially. It has a LONGER shelf LIFE due to the INCORPORATION of the antisense GENES within its genome.
105.

Delay in the ripening of tomato is due to_____(a) virulent gene(b) antisense PG(c) antibiotic resistance gene(d) mutationThe question was asked in semester exam.Question is from Biotechnology Applications in section Biotechnology and its Applications of Biology – Class 12

Answer»

Right answer is (b) antisense PG

For explanation: PG stands for an enzyme called polygalacturonase which is responsible for pectin DEGRADATION leading to early ripening. Antisense PG INHIBITS the FORMATION of polygalacturonase thus DELAYING the PROCESS of ripening.

106.

Which agriculturally important bacterial gene is isolated from Rhizobium?(a) Nif gene(b) E-gene(c) F-gene(d) T-DNAThis question was posed to me in an online interview.The above asked question is from Biotechnology Applications topic in chapter Biotechnology and its Applications of Biology – Class 12

Answer»

Correct ANSWER is (a) Nif gene

For explanation: Nif GENES are a SET of genes that are involved in ATMOSPHERIC NITROGEN fixation. They are isolated from a bacterium Rhizobium. It is a Gram-negative bacterium which symbiotically assists the leguminous plants and fix nitrogen.

107.

RNAi takes place in all prokaryotic organisms.(a) True(b) FalseThis question was posed to me in an international level competition.My question is taken from Biotechnology Applications in division Biotechnology and its Applications of Biology – Class 12

Answer»

Right answer is (b) False

Easy explanation: RNAi is a series of processes that involve the silencing of MRNA sequence which prevents TRANSLATION of that sequence. It takes place in all EUKARYOTES like animals, PLANTS, and FUNGI. It is not seen in prokaryotes.

108.

Sense and antisense RNA forms _____(a) ds RNA(b) ss RNA(c) ds DNA(d) ss DNAThis question was addressed to me during an interview for a job.The above asked question is from Biotechnology Applications topic in chapter Biotechnology and its Applications of Biology – Class 12

Answer»

The correct choice is (a) ds RNA

The best I can EXPLAIN: The sense and antisense RNA FORMS ds RNA. The sense strand has SEQUENCE same as that of mRNA which codes for a functional protein while the antisense strand CONSISTS of complementary nucleotides as that of the sense strand.

109.

Formation of _______ initiates RNAi process.(a) ds DNA(b) ssDNA(c) ss RNA(d) ds RNAThe question was posed to me in exam.I need to ask this question from Biotechnology Applications topic in chapter Biotechnology and its Applications of Biology – Class 12

Answer»

The correct choice is (d) DS RNA

For explanation: Formation of ds RNA initiates the RNAi process. RNAi is a series of processes that involve the SILENCING of mRNA sequence which prevents translation of that sequence. It is a widely USED technique used for GENE silencing.

110.

Sense and antisense RNA are _______ to each other.(a) similar(b) same(c) complementary(d) differentThe question was asked by my school principal while I was bunking the class.This key question is from Biotechnology Applications topic in section Biotechnology and its Applications of Biology – Class 12

Answer»

Right CHOICE is (c) complementary

To EXPLAIN: The strand having sequence exact same to that of MRNA is called SENSE strand. The antisense strand consists of a complementary sequence that of transcribed mRNA. Antisense strand serves the template for the TRANSCRIPTION, thus sense and antisense RNA are complementary to each other.

111.

In RNAi, the DNA is introduced into a host plant in such a way that it produces both ________(a) complementary DNA and mRNA(b) mRNA and sense RNA(c) sense and antisense RNA(d) DNA and RNAI got this question in homework.I'm obligated to ask this question of Biotechnology Applications topic in section Biotechnology and its Applications of Biology – Class 12

Answer»

The correct answer is (C) sense and antisense RNA

Explanation: RNAi stands for RNA INTERFERENCE. RNAi is a method involving the silencing of specific mRNA due to the binding of COMPLEMENTARY dsRNA. In RNAi, the DNA is introduced into a host plant in such a way that it produces both sense and antisense RNA.

112.

_______ prevents the translation of the mRNA.(a) Enhancing(b) Knock out(c) Clearing(d) SilencingI got this question in my homework.This is a very interesting question from Biotechnology Applications topic in section Biotechnology and its Applications of Biology – Class 12

Answer»

Correct choice is (d) Silencing

Easiest explanation: Silencing prevents the TRANSLATION of the MRNA. It regulates the expression of a GENE by PREVENTING its expression. This is used in the RNAi technique. It is a METHOD used for knowing the function of the gene.

113.

A method involving silencing of specific mRNA due to the binding of complementary dsRNA is called ________(a) Electrophoresis(b) RNAi(c) rDNA technology(d) DNAiI have been asked this question in semester exam.I would like to ask this question from Biotechnology Applications topic in portion Biotechnology and its Applications of Biology – Class 12

Answer»

The correct choice is (B) RNAi

The explanation: RNAi is a METHOD involving the silencing of specific mRNA due to the binding of complementary dsRNA. It CONSISTS of various processes. It acts as a defense mechanism against VIRUSES and transposable ELEMENTS.

114.

_________ is mobile genetic elements.(a) Viruses(b) Bacteria(c) Transposons(d) Insertion sequencesThis question was addressed to me in an internship interview.This interesting question is from Biotechnology Applications in division Biotechnology and its Applications of Biology – Class 12

Answer»

Correct choice is (c) Transposons

Best explanation: Transposons are mobile genetic elements and are ALSO known as jumping genes. These are the sequences of DNA that can MOVE from ONE position to another within the GENOME. They were first discovered by Barbara McClintock in maize plants.

115.

______ vectors are used for the introduction of nematode specific genes in the host.(a) Bacterial(b) Viral(c) Agrobacterium(d) Meloidogyne incognitiaI had been asked this question in an interview for job.The above asked question is from Biotechnology Applications in portion Biotechnology and its Applications of Biology – Class 12

Answer»

The correct choice is (c) AGROBACTERIUM

For explanation I would say: Agrobacterium vectors are used for the INTRODUCTION of nematode specific genes in the HOST. This was uses for RNAi technique. INITIALLY, the host plant used was a tobacco plant.

116.

RNAi stands for ______(a) RNA intersection(b) RNA insertion(c) RNA intercom(d) RNA interferenceThis question was addressed to me during an interview.My question is taken from Biotechnology Applications topic in portion Biotechnology and its Applications of Biology – Class 12

Answer»

Correct OPTION is (d) RNA INTERFERENCE

To explain I would say: RNAi stands for RNA interference. It is a series of PROCESSES in which short RNA sequences are used to recognize and MANIPULATE complementary nucleic acids. These short RNA sequences consist of 20-30 nucleotides. It protects against viruses and transposable elements.

117.

Which one is the method of cellular defense in animals and plants?(a) Recombinant DNA technology(b) RNA interference(c) Immune response(d) MutationThe question was asked in an interview for internship.My question is based upon Biotechnology Applications in section Biotechnology and its Applications of Biology – Class 12

Answer»

The correct answer is (b) RNA interference

To elaborate: RNA interference is one of the cellular defense mechanisms FOUND in animals and plants. It defends against VIRUSES and TRANSPOSABLE elements. In this METHOD, short RNA STRETCHES are used (20-30 nucleotides).

118.

Which nematode infects the roots of tobacco plants?(a) Meloidogyne incognitia(b) Ditylenchus dipsaci(c) Xiphinema index(d) Nacobbus aberransI have been asked this question during an interview.My question comes from Biotechnology Applications in section Biotechnology and its Applications of Biology – Class 12

Answer»

Correct CHOICE is (a) Meloidogyne incognitia

Explanation: Meloidogyne incognitia is the NEMATODE that infects the roots of tobacco plants. It is also known as ‘root-knot nematode’. It is a plant-parasitic nematode. It affects both monocots as WELL as DICOT plants.

119.

Ti plasmid is found in ________(a) Nitrosomonas(b) Escherichia(c) Agrobacterium(d) BacillusI have been asked this question in a job interview.This question is from Biotechnology Applications in section Biotechnology and its Applications of Biology – Class 12

Answer»

The correct OPTION is (c) Agrobacterium

The explanation is: Ti plasmid i.e. tumor-inducing plasmid is found in Agrobacterium tumefaciens. This plasmid acts as a VECTOR for the Bt GENE. This Ti plasmid consists of T DNA. This T DNA, when expressed in plants, leads to tumor FORMATION.

120.

Toxic content of B. thuringiensis is _______ in nature.(a) protein(b) steroid(c) amino acid(d) alkaloidThe question was asked in an international level competition.I'd like to ask this question from Biotechnology Applications in section Biotechnology and its Applications of Biology – Class 12

Answer» RIGHT answer is (a) PROTEIN

For EXPLANATION I would say: The toxic content of B. thuringiensis consists of protoxins. These protoxins are protein in nature. These are cry proteins that kill the insect by forming crystals in its GUT. Protoxins are endotoxins which can be used as BIOPESTICIDES.
121.

Bt gene which produces a protein toxic to insect larvae is ______(a) try(b) pry(c) ary(d) cryThe question was asked by my college professor while I was bunking the class.The origin of the question is Biotechnology Applications in chapter Biotechnology and its Applications of Biology – Class 12

Answer»

The correct choice is (d) cry

For explanation: The Bt gene produces a protein TOXIC to insect LARVAE codes for cry protein. Cry protein is a toxic protein encoded by cry gene. This protein is toxic to VARIOUS insects and is produced by B. thuringiensis BACTERIA.

122.

A gall producing gene in Agrobacterium tumefaciens is _______(a) cry gene(b) nif gene(c) T DNA(d) beta geneThis question was posed to me in unit test.This interesting question is from Biotechnology Applications in section Biotechnology and its Applications of Biology – Class 12

Answer»

Right option is (C) T DNA

The explanation: T DNA is the transfer-DNA. The GALL producing gene in AGROBACTERIUM tumefaciens is present in T DNA. It is the DNA of TI (tumor-inducing) plasmid. It is also found in A. rhizogenes.

123.

To which cells does the activated Bt toxin bind?(a) Neuroglial cells(b) Lymphocytes(c) Skeletal muscle cells(d) Epithelial cellsI have been asked this question in an online interview.My enquiry is from Biotechnology Applications in chapter Biotechnology and its Applications of Biology – Class 12

Answer» RIGHT option is (d) Epithelial cells

The best I can explain: The activated Bt TOXIN BINDS to the midgut epithelial cells. These cells line the INNER and outer surface of our BODIES such as skin, alimentary canal, urinary tract, and organs.
124.

The Bt toxin does not kill the Bacillus because the Bt toxin protein exist as inactive ________(a) lipid(b) protoxins(c) protein(d) carbohydrateThe question was asked during an interview.My query is from Biotechnology Applications in portion Biotechnology and its Applications of Biology – Class 12

Answer» CORRECT answer is (b) protoxins

The explanation: The Bacillus is not killed by its own BT TOXIN because this Bt toxin protein EXISTS as inactive protoxins in it. This toxin converts into an active form in an ALKALINE pH. This later on form crystals which lead to the death of that insect.
125.

The protein crystals of B. thuringiensis contain toxic _______ protein.(a) bactericidal(b) fungicidal(c) insecticidal(d) antibioticThis question was posed to me during an interview.My question is taken from Biotechnology Applications in portion Biotechnology and its Applications of Biology – Class 12

Answer»

Correct answer is (c) insecticidal

For explanation I WOULD SAY: The protein crystals of B. thuringiensis contain toxic insecticidal protein. This protein acts against INSECTS such as lepidopterans. They cause crown GALL DISEASE to plants.

126.

The gene which was used to produce insect-resistant cotton plant was taken from ______(a) Anabaena azollae(b) Agrobacterium tumefaciens(c) Bacillus thuringiensis(d) Bacillus subtilisThis question was posed to me in homework.Query is from Biotechnology Applications topic in division Biotechnology and its Applications of Biology – Class 12

Answer» CORRECT choice is (c) Bacillus thuringiensis

For explanation I WOULD say: The gene from Bacillus thuringiensis was USED to PRODUCE insect-resistant plants. This gene was later on inserted into a bacterium called Agrobacterium tumefaciens which act as a VECTOR for the Bt gene.
127.

Insect resistance transgenic cotton has been developed by inserting a piece of DNA from ______(a) an insect(b) wild relative of cotton(c) a virus(d) a soil bacteriumThis question was addressed to me in an online interview.My query is from Biotechnology Applications topic in section Biotechnology and its Applications of Biology – Class 12

Answer»

The correct option is (d) a SOIL bacterium

For explanation I would say: Insect resistance TRANSGENIC cotton has been developed by inserting a piece of DNA from a soil bacterium. The bacterium used in this is Agrobacterium tumefaciens. This organism is responsible for causing crown GALL disease in an insect.

128.

Bt cotton is a _______(a) cloned plant(b) transgenic plant(c) hybrid plant(d) mutated plantI got this question in quiz.The doubt is from Biotechnology Applications topic in chapter Biotechnology and its Applications of Biology – Class 12

Answer» RIGHT choice is (b) transgenic plant

Explanation: Bt cotton is an example of a transgenic plant. These are plants WHOSE DNA is modified USING genetic engineering TECHNIQUES (rDNA technology). This plasmid is used as a vector to insert a foreign gene within a plant.
129.

Some strains of Bacillus thuringiensis can kill certain insects such as _______(a) lepidopterans(b) scorpion(c) fruit fly(d) dragonflyI have been asked this question in a national level competition.The origin of the question is Biotechnology Applications topic in section Biotechnology and its Applications of Biology – Class 12

Answer» RIGHT option is (a) lepidopterans

Explanation: Lepidopterans are an order of insects consisting of moths and BUTTERFLIES. Some insects of this order can be killed by the bacteria BACILLUS thuringiensis. A few EXAMPLES of lepidopterans are tobacco budworm, armyworm, bettles, etc.
130.

Bt toxin is produced by a bacterium called ______(a) Bacillus thuringiensis(b) Bacillus anthracis(c) Bacillus thermophilus(d) Bacillus subtilisThe question was asked during an interview for a job.My enquiry is from Biotechnological Applications in Agriculture in section Biotechnology and its Applications of Biology – Class 12

Answer»

Right answer is (a) Bacillus thuringiensis

To explain I would say: Bt STANDS for Bacillus thuringiensis. It is a bacterium that PRODUCES Bt toxin. In plants the gene of this toxin provides resistance against insects, THUS there is no NEED for spraying insecticides on plants.

131.

________ was the first transgenic crop.(a) Potato(b) Brinjal(c) Cotton(d) TobaccoThis question was addressed to me in final exam.My doubt is from Biotechnological Applications in Agriculture in portion Biotechnology and its Applications of Biology – Class 12

Answer»

Correct option is (d) TOBACCO

For EXPLANATION: The first transgenic crop (GMO) was tobacco. It was DEVELOPED in the year 1983. It was first commercially released in China in 1992. It was a virus-resistance PLANT.

132.

GMOs can minimize the use of fertilizers.(a) True(b) FalseThe question was posed to me by my college director while I was bunking the class.My question is taken from Biotechnological Applications in Agriculture topic in chapter Biotechnology and its Applications of Biology – Class 12

Answer»

Right answer is (a) True

To explain I would say: GMOs can minimize the use of fertilizers. GMO’s itself has genes that can FIGHT against the INFECTIOUS ORGANISM’s thus GIVING HIGH yield. It also has high nutritional content.

133.

GM rice is enriched with vitamin _____(a) K(b) D(c) A(d) EThis question was posed to me in a job interview.My query is from Biotechnological Applications in Agriculture in portion Biotechnology and its Applications of Biology – Class 12

Answer»

Correct option is (c) A

Easy explanation: GM RICE is enriched with vitamin A. it is ALSO known as golden rice. Gm rice produces BETA carotene which is a precursor of vitamin A. Vitamin A is IMPORTANT for growth and development and for GOOD vision.

134.

A GM crop is _______(a) mature crop(b) infected crop(c) raised to green manure(d) transgenic cropThe question was asked by my school principal while I was bunking the class.My question is from Biotechnological Applications in Agriculture topic in chapter Biotechnology and its Applications of Biology – Class 12

Answer»

The correct OPTION is (d) transgenic crop

The explanation: GM stands for Genetically Modified. A GM crop is a transgenic crop in which the GENES of the DESIRED crop are changed by manipulations USING various METHODS. This method gives a high yield without waste of crops.

135.

An example of abiotic stress is ________(a) pest(b) insect(c) drought(d) vectorsThe question was asked in final exam.This intriguing question originated from Biotechnological Applications in Agriculture in chapter Biotechnology and its Applications of Biology – Class 12

Answer»

Right answer is (c) drought

To EXPLAIN: Abiotic stress is the negative IMPACT of the non-living factors on the environment. Stress is created when this non-living factor influences the environment beyond its normal RANGE. Examples of abiotic stress are drought, cold, HEAT, salt, ETC.

136.

An important objective of biotechnology in the area of agriculture is ________(a) to decrease seed number(b) to produce pest-resistant varieties of plants(c) to increase phosphorous, nitrogen production(d) to reduce the number of plantsThe question was asked in an online quiz.The origin of the question is Biotechnological Applications in Agriculture topic in portion Biotechnology and its Applications of Biology – Class 12

Answer»

Right option is (B) to produce pest-resistant varieties of PLANTS

To explain I WOULD say: An important objective of biotechnology in the area of agriculture is to produce pest-resistant varieties of plants. This will help in less wasting of CROPS with high YIELD. It will also give more products in less time and space.

137.

GMO stands for _______(a) Genetically Matured Organisms(b) Genetically Mental Organs(c) Genetically Modified Organisms(d) Genetically Mutant OrganismI got this question during an interview for a job.My query is from Biotechnological Applications in Agriculture topic in portion Biotechnology and its Applications of Biology – Class 12

Answer»

The CORRECT option is (c) GENETICALLY Modified Organisms

Explanation: GMO stands for Genetically Modified Organisms. The genes of these organisms have been manipulated to OBTAIN the desired product. GMO can be a plant, bacteria, FUNGI or animal.

138.

Who led the green revolution in India?(a) Neel Kranti(b) Verghese Kurien(c) M.S. Swaminathan(d) M.K. GandhiThis question was posed to me at a job interview.I'd like to ask this question from Biotechnological Applications in Agriculture in chapter Biotechnology and its Applications of Biology – Class 12

Answer» CORRECT choice is (C) M.S. Swaminathan

To explain: The green revolution in India was founded by M.S. Swaminathan. It was a part of the green revolution led by NORMAN Borlaug. It focused on increasing the agricultural yield, especially in states of Haryana, PUNJAB and Uttar Pradesh.
139.

Who was the leader of the green revolution?(a) Verghese Kurien(b) Neel Kranti(c) Napoleon Bonaparte(d) Norman BorlaugThe question was asked in examination.This interesting question is from Biotechnological Applications in Agriculture in chapter Biotechnology and its Applications of Biology – Class 12

Answer»

The correct CHOICE is (d) Norman Borlaug

Easiest explanation: The Green REVOLUTION is related to Norman Borlaug. Neel Kranti is the name assigned to the blue revolution by the INDIAN government. Napoleon Bonaparte led the French revolution while the “Father of WHITE Revolution” is VERGHESE Kurien.

140.

What is the green revolution related to?(a) Milk(b) Agriculture(c) Water(d) AnimalsThis question was posed to me in an online interview.The query is from Biotechnological Applications in Agriculture in portion Biotechnology and its Applications of Biology – Class 12

Answer»

Correct choice is (b) Agriculture

The BEST explanation: The green revolution is related to agriculture. It occurred between 1950-1960 and MAINLY focused on increasing agricultural PRODUCTION. It started in MEXICO.

141.

What does the second critical research area of biotechnology provide?(a) Optimum conditions for the catalyst(b) The pure form of catalyst(c) An impure form of catalyst(d) Purification of productsI have been asked this question in quiz.This key question is from Biotechnological Applications in Agriculture in section Biotechnology and its Applications of Biology – Class 12

Answer» RIGHT option is (a) Optimum conditions for the catalyst

The explanation: There are three critical research AREAS in BIOTECHNOLOGY. They are 1. It provides the best catalyst in the form of an IMPROVED organism (transformants) usually a MICROBE or pure enzyme. 2. It creates optimum conditions for the catalyst by the method of genetic engineering. 3. Purification of products by downstream processing.