InterviewSolution
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InterviewSolution
This section includes InterviewSolutions, each offering curated multiple-choice questions to sharpen your knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
Crystals of Bt toxin produced by some bacteria do not kill the bacteria themselves because |
| Answer» Correct Answer - C | |
| 2. |
Crystals of Bt toxin produced by some bacteria do not kill the bacteria themselves becauseA. Bacteria are resistantto the toxinB. Toxin is immatureC. Toxin is inactiveD. Bacteria encloses toxin in a special sac |
| Answer» Correct Answer - C | |
| 3. |
What could be the possible harm of using antibiotic resistant gene as a selectable marker in plasmid, used for gene transfer in production of GM food? (A) GM food contains the enzyme produced by the antibiotic resistance gene that was used during gene tranfer in genetic engineering. This could cause allergies since it is a foreign protein. (B) The bacteria present in the alimentary canal of the humans could take up the antibiotic resistance gene present in GM food. (C) The bacteria in the human alimentary canal would then become resistant to the concerned antibiotic. (D) The transgene may be transferred through pollen to their wild relattves and make weeds more persistant and damaging.A. A onlyB. Both B and CC. A,B and CD. A,B,C and D |
| Answer» Correct Answer - C | |
| 4. |
The bacterum Bacillus thuringiensis is widely used in contemporary biology asA. BioweaponB. BioinsecticideC. BioweedicideD. Indicator of water pollution |
| Answer» Correct Answer - B | |
| 5. |
Read the following four statements (A-D) A. The first transgenic buffalo, Roise produced milk which was human alpha-lactalbumin enriched B. Restriction enzymes are used in isolation of DNA from other macromolecules C. Downstream processing is one of the step of rDNA technology D. Disarmed pathogen vectors are also used in transfer of rDNA into the host which of the two statements have mistakes ?A. (A) & (C)B. (A) & (B)C. (B) & (C)D. (C ) & (D) |
| Answer» Correct Answer - B | |
| 6. |
RNA interference which is employed in making tobacco plant resistant to Meloidegyne incognita is essentially involved in preventing the process of:A. translation of mRNAB. transcriptionC. replication of DNAD. splicing of hnRNA |
| Answer» Correct Answer - A | |
| 7. |
Which of the following may be considered as an application of biotechnology?A. BioremediationB. Waste treatmentC. Energy productionD. All of these |
| Answer» Correct Answer - D | |
| 8. |
Which of the following is not included under the application of biotechnology ?A. Genetically modified cropsB. Processed foodC. Wast treatment and energy productionD. None of the above |
| Answer» Correct Answer - D | |
| 9. |
An important objective of biotechnology in agriculture section is toA. to produce pest resistant varieties of plantsB. to increase the nitrogen contentC. to decrease the seed numberD. to increase the plant weight |
| Answer» Correct Answer - A | |
| 10. |
Product of biotechnology isA. transgenic crops (GM crops)B. humulinC. biofertiliserD. All of the above |
| Answer» Correct Answer - D | |
| 11. |
Kohler and Milstein developed a method in biotechnology for the production ofA. myelomasB. steroid conversionC. monoclonal antibodiesD. immobilised enzymes |
| Answer» Correct Answer - C | |
| 12. |
The problem of blindness in poor countries can be taken care of by using the following:A. golden riceB. wheatC. gramD. pea |
| Answer» Correct Answer - A | |
| 13. |
Match list I with List II and select the correct option A. a-2,b-4,c-1,d-5,e-3B. a-2,b-4,c-5,d-1,e-3C. a-4,b-3,c-5,d-2,e-3D. a-3,b-4,c-5,d-1,e-2 |
| Answer» Correct Answer - C | |
| 14. |
A transgenic food crop which may help in solving the problem of night blindness in developing countries isA. Golden riceB. Br soybeanC. Flavr savr tomatoD. Starlink maize |
| Answer» Correct Answer - A | |
| 15. |
You have identified a useful gne in bacteria. Make a flow chart of the steps that you would follow to trasnfer this gene to a plant. |
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Answer» After identifying a useful gene in bacteria, following steps should be followed (i) Isolation of useful gene using restriction endonucleases `downarrow` (ii) Ttransferrin g the gen e to a suitable vector to create a recombinant DNA molecule `downarrow` (iii) Trasnfer of these recombinant DNA molecuels to the target cells. `downarrow` (iv) Screening of cells for transformation `downarrow` (v) Selection of transformed cells `downarrow` (vi) Regeneration of platns from the trasnformed cells to get transgenic plants. |
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| 16. |
A plant expressing a gene from another organisms isA. transgenicB. cloneC. transformedD. somaclonal variant |
| Answer» Correct Answer - A | |
| 17. |
The principle patending pirates areA. USB. JapanC. GermanD. All of these |
| Answer» Correct Answer - D | |
| 18. |
In RNAi, genes are silenced usingA. ss DNAB. ds DNAC. ds RNAD. ss RNA |
| Answer» A nematode Meiodegyne incognitia infects the roots of tobaccoo plants which reduces the production of tobaccoo. It can be prevented by using RNA interference process, which is checked by silencing of specific mRNA due to a complementary dsRNA. dsRNA binds and prevents the translation of mRNA (silencing). | |
| 19. |
Mention the chemical change proinsulin undergoes to be able to act as mature insulin |
| Answer» Removal of C chain by processing A few important products o fbiotechnology are alcohols organic acids enzymes vitamins steroids monochlonal antibodies insulin etc | |
| 20. |
(a) write name of the first transgenic crop in india (B) insulin is extracted formf which microorganisnm? (C ) which enzyme is most commonly used for the crop improvement in genetic enginering? |
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Answer» (a) A transgenic crop is a crop that contains a transgene foreign gene is introduced andy stably integrated in to the host DNA The name of first transgenic crop produced in india is Tobacco (Nicteria -tabacum) (b) Insulin producing genes form the human being have been introduced in to the bacteria Escherichia coli (E coli) The genetically moidified bacterium E coli produces insulin called Humulin This insulin is extract ed form E coli for clinical use (c ) The enzyme restriction endonucleases is most commony used for crop imporvement in genetic engineering |
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| 21. |
Presence of which of the following in proinsulin makes it different from insulinA. A - peptideB. B - peptideC. C - peptideD. disulphide bonds |
| Answer» Correct Answer - C | |
| 22. |
Human insulin is being commercially produced from a transgenic species ofA. RhizobiumB. SaccharomycesC. EscherichiaD. Mycobacterium |
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Answer» Correct Answer - C In 1983 an American compnay Eli Lily produced the first genetically engineered insulin by first synthesising two DNA sequences corresponding to `alpha and beta` insulin chains. The two DNA sequences or genes were made to fuse with plasmids of Escherichia coli and later allowed to form insulin chains. |
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| 23. |
Human insulin is being commercially produced from a transgenic species of :A. SaccharomycesB. E.coliC. MycobacteriumD. Agrobacterium |
| Answer» Correct Answer - B | |
| 24. |
Which peptide chain is removed during maturation of proinsulinA. D chainB. C chainC. B ChainD. A chain |
| Answer» Correct Answer - B | |
| 25. |
Which genes encode the protein to control bollworms infection in cotton plants?A. c,y IIAbB. cry IAcC. Both (1) & (2)D. cry IAb |
| Answer» Correct Answer - C | |
| 26. |
Some of the steps involved in production of humulin are given below. Choose the correct sequence (i) Synthesis of insulin gene artificially (ii) Culturing recombinant E. coli in bioreactors (iii) Purification of humulin (iv) Insertion and human insulin gene into plasmid (v) Introduction of recombinant plasmid into E. coli (vi) Extraction of recombinant gene product from E. coli.A. ii, i, iv, iii, v, viB. i, iii, v, vi, ii, ivC. I,iv,v,ii,vi,iiiD. iii,v,ii,I,vi,iv |
| Answer» Correct Answer - C | |
| 27. |
The two polypeptides of human insulin are linked together byA. phosphodiester bondsB. covalent bondsC. disulphide bridgesD. hydrogen bonds |
| Answer» Correct Answer - C | |
| 28. |
With respect to understanding diseases, discuss the importance of transgenic animal models. |
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Answer» Transgenic animals are important in the following fields (i) They are being used in basic science research to elucidate the role of genes in the development of diseasses like cancer cystic fibrosis, rheumatoid arthritis and development of diseases like cancer cystic fibrosis, rheumatioid arthrits and alzheimer . (ii) They are valuable tools in the drug development process itself. (iii) They can produce medicines or human proteins (insulin growth hormone etc ) in large qu antities. (vi) Transgenics can be a source of trasnplant organs as well. |
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| 29. |
Animal that have their DNA manipulated to possess and express an extra (foreign) gene are known as-----A. Foreign animalsB. Superior animalsC. Transgenic animalsD. Elevated animals |
| Answer» Correct Answer - C | |
| 30. |
Which of the following is correct statement w.r.t. animal insulin?A. It is just as effective as human insulin over prolonged timeB. It does not elicit immune response everC. It can be orally administrated to diabetic peopleD. It was extracted from pancreas of slaughtered cattle and pigs |
| Answer» Correct Answer - D | |
| 31. |
Meloidegyne is classified under which phuylum of animal kingdom? |
| Answer» Aschelminthes (Nematoda) | |
| 32. |
RNAi takes place in all prokaryotic organisms. State True/False. |
| Answer» False, occurs in eukaryotes. | |
| 33. |
A : RNAi takes place in all eukaryotic organisms as a method of cellular defense. R : Complementary dsRNA molecule binds to specific mRNA and prevents its translation (silencing).A. If both Assertion & Reason are true and the reason is the correct explanation of the assertion, then mark (1 )B. If both Assertion & Reason are true but the reason is not the correct explanation of the assertion, then mark (2).C. If Assertion is true statement but Reason is false, then mark (3).D. If both Assertion and Reason are false statements, then mark (4). |
| Answer» Correct Answer - B | |
| 34. |
A : Bt toxin are protein crystals containing insecticidal protein R : B. thuringiensis forms these protein crystals throughout continuously during their growth period.A. If both Assertion & Reason are true and the reason is the correct explanation of the assertion, then mark (1 )B. If both Assertion & Reason are true but the reason is not the correct explanation of the assertion, then mark (2).C. If Assertion is true statement but Reason is false, then mark (3).D. If both Assertion and Reason are false statements, then mark (4). |
| Answer» Correct Answer - C | |
| 35. |
Bt toxin is obtained from:A. prokaryotesB. eukaryotesC. Both (a) and (b)D. None of these |
| Answer» Correct Answer - A | |
| 36. |
Match the following Columns. `{:(,"Column I",,,"Column II"),(A.,"Golden rice",,1.,"Armyworm"),(B.,"Bt toxin",,2.,"Rich in vitamin - A"),(C., "RNAi",,3.,"Cry protein"),(D.,"Lepidopterans",,4.,"Gene silencing"):}`A. `{:(A,,B,,C,,D),(2,,3,,4,,1):} `B. `{:(A,,B,,C,,D),(3,,4,,1,,2):} `C. `{:(A,,B,,C,,D),(4,,1,,2,,3):} `D. `{:(A,,B,,C,,D),(2,,1,,3,,4):} ` |
| Answer» Correct Answer - A | |
| 37. |
What is true about Bt toxin?A. Bt protein exists as active toxin in the BacillusB. The activated toxin enters the ovaries of the pest to sterilise it and thus prevent its multiplicationC. The concerned Bacillus has antitoxinsD. The inactive protoxin gets converted into active form in the insect gut. |
| Answer» Correct Answer - D | |
| 38. |
Transgenic plants are the ones:A. Generated by introducing foreign DNA into a cell and regenerating a plant from that cellB. Produced after protoplast fusion in artificial mediumC. Grown in artificial medium after hybridization in the field.D. Produced by a somatic embryo in artificial medium. |
| Answer» Correct Answer - A | |
| 39. |
Transgenic plants are the ones:A. generated by introducing foreign DNA into a cellB. produced after protoplast fusion in artificall mediumC. grown in artificial medium after bybridiztion in the fieldD. produced by a somatic embroyo in artifical medium |
| Answer» Correct Answer - A | |
| 40. |
Polyethylene glycol method is used forA. Biodiesel productionB. Seedless fruit productionC. Energy production from sewageD. Gene transfer without a vector |
| Answer» Correct Answer - D | |
| 41. |
Which of the following statements about Bacillus thuringiensis are correct? A. One of the toxin produced by the bacteria is thurioside, which is active against different groups of insect larvae. B. The toxin accumulates inside the bacteria during sporulation. C. Upone ingestion by susceptible insects they are converted into active form and kill them by inhibition of ion transport in the midgut. D. The proteins encoded by the gene Cry II Ab contrls com borer.A. B onlyB. A and BC. A, B and CD. A, B, C and D |
| Answer» Correct Answer - C | |
| 42. |
Golden rice I. It is transgenic variety of rice. II. It contains a good quality of ` beta ` - carotene (pro - vitamin -A) IV. The grains of the rice are yellow in colour due to ` beta` - carotene. The rice is commonly called golden rice. Which of the statements given above are correct.A. I, II and IIIB. II, III and IVC. I, III and IVD. I, II, III and IV |
| Answer» Correct Answer - D | |
| 43. |
Which of thedollowing genetically engineered microbe is used in scavening of oil spills by digesting hydocarbons of crude oil?A. Pseudomonas fluorescenceB. Rhizobium melilotiC. Pseudomonas pitidaD. Trichoderma |
| Answer» Correct Answer - C | |
| 44. |
Which of the following is not an application of genetic engineering in plants?A. Nitrogen fixationB. DNA vaccinesC. Resistance to glyphosateD. Production of insecticidal proteins in plants |
| Answer» Correct Answer - B | |
| 45. |
Which one of the following bacteria has found extensive use in genetic engineering work in plants?A. Clostridium septicumB. Xanthomonas citriC. Bacillus coagulensD. Agrobacterium tumefacriens |
| Answer» Correct Answer - D | |
| 46. |
A: Indian Government has set up organisation such as GEAC (Genetic Engineering Approval Committee), which will make decisions regarding the validity of GM research and safety of introducting GM organisms for public services. R: Genetic modification of organisms can have unpredictable results when such organism are introduced into the ecosystem.A. If both Assertion & Reason are true and the reason is the correct explanation of the assertion, then mark (1 )B. If both Assertion & Reason are true but the reason is not the correct explanation of the assertion, then mark (2).C. If Assertion is true statement but Reason is false, then mark (3).D. If both Assertion and Reason are false statements, then mark (4). |
| Answer» Correct Answer - A | |
| 47. |
Which is used in production of insulin by genetic engineering ?A. Escherichia coliB. MycobacteriumC. Both (a) and (b)D. None of the above |
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Answer» Correct Answer - A In 1983, an American company Eli Lily and company synthesised artified insulin with the help of plasmids of Escherichia coli. It was named as humulin. |
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| 48. |
Expression of which enzyme is blocked in Flavr savr tomato ?A. Carotene sataraseB. ColepteronsC. Diacetyl transferaseD. polygalacturonase |
| Answer» Correct Answer - D | |
| 49. |
Which one of the following bacteria is used for production of transgenic plants ?A. Escherichia coliB. PseudomonasC. Staphylococcus aureusD. Agrobacterium tumefaciens |
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Answer» Correct Answer - D Plant cells do not have endogeneous plasmids. The plasmid vectors used for plant cell transformation are mostly based on Agrobacterium tumefaciens Ti plasmid. These are plant pathogenic Gram positive soil bacteria which cause crown gall disease of dicot plants. |
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| 50. |
Bt-cotton is resistant to:A. insectsB. herbicidesC. salt resistantD. drought resistant |
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Answer» Correct Answer - A Bt cotton is produces by the insertion of insect resistance genes from bacterium Bacillus thuringiensis to cotton plant. This gene produces an insect resistant protein toxin crystals, called parasporal body (delta endotoxin ) that can act as a microbial insecticide for specific insect groups. |
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