InterviewSolution
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InterviewSolution
This section includes InterviewSolutions, each offering curated multiple-choice questions to sharpen your knowledge and support exam preparation. Choose a topic below to get started.
| 51. |
Bt cotton is resistant againstA. lepidopteransB. colepteransC. dipteransD. All of these |
| Answer» Correct Answer - D | |
| 52. |
The strategy used to prevent the nematode infection in the roots of tobacoo plant in called:A. use of agrochemicalsB. Bt toxin geneC. gene mutationD. RNA interference |
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Answer» Correct Answer - D Nematode is a group of organisms which parasitise a large number of plants and animals including human beings. One of the most common nematodes (Meloidegyne incognita ) infects the roots of tobacco plants and causes a great loss by causing reduction in yield. This infectation was prevented by using a novel strategy which was based on the process of RNA interferance (RNAi ) |
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| 53. |
Genetically modified tobacoo plant with Bt gene is resistant toA. BollwormsB. HornwormsC. HookwormsD. Roundworms |
| Answer» Correct Answer - A | |
| 54. |
What was the speciality of the milk produced by the transgenic cow Rosie ? |
| Answer» The first transgenic cow rosie produced human protein enriched milk that contained the human alhpa lactalbumin (2.4 gms per liter) This milk was more balanced and good for human babies as compared ot natural cow milk | |
| 55. |
Which gene was introduced in the first transgenic cow?A. Human ` alpha ` - lactalbuminB. ` alpha - 1 ` - antitrypsinC. ` beta - ` - antitrypsinD. Cry IAc |
| Answer» Correct Answer - A | |
| 56. |
Gene therapy is an attempt to correct a genetic defect by providing a normal gene into the individual. By this the normal function can be restored. An alternate method would be to provide the gene product (protein/enzyme) known as replacement thereapy. which would also restore the function.which in your opinion is a better option? Give reason for your answer. |
| Answer» Gene therapy would be a better option because it has the potential to completely cure the patient. It is because the correct gene once introduced in the patient. Can continue to produce the correct protein enzyme Enzyme therapy does not offer permanent cure as it needs to be given to the patient on regular basis. it is also more expensive. | |
| 57. |
Transgenic animals are the animals are the animals in which foreign gene is expressed. Such animals can be used to study the fundamental biological process, phenomenon as well as for producing products useful for mankind. Give one example for each type. |
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Answer» Transgenic animals are the animals in which a foreign genes are expressed . Such animals can be used to stuty the fundamental biological process/phenomenon e.g., by using modeal organisms like mouse we can determine how genes areregulated (gene regularion) how they affect the normal functions of the body and its development etc. Trangenic animals are also used for producing products useful for mankind e.g., Transgenic cow (rosie). which produced human protein enriched milk (2.4g/L). the milk contained the human alpha-lactabumin and was nutritionally a more balanced product for human babies than natural cow-milk. |
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| 58. |
How did Eli Lilly synthesis the human insulin ? Mention one difference between this insulin and the one produced by the human pancreas. |
| Answer» Eli lilly company prepared two DNA sequences coding for chain A & B of human insulin nd introduces it into the palsmids of E coli which was utilise to produce finsulin . Insulin form an animal source causes allergy in some patints in humans insullin in synthesised as a prophormine which has one extrao strech called c peptide | |
| 59. |
Name the five key tools for accomplishing the tasks of recombinant DNA technology. Also mention the functions of each tool. |
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Answer» The key tools for accomplising the tasks of recombinant DNA technology with their functions are mentioned below. (i) Restriction endocleases for cutting the desrired DNA at desired places. (ii) Gel electrophoresis for separating the desired DNA fragements. Ligase enzyme for creating recombinant DNA molecule (iii) Ligase enzyme for creating recombinant DNA molecule. (iv) DNA delivery system like electroporation, microinjection, gene gun method, etc. (v) Competant host (usually bacteria yeast) to take up the recombinant DNA. |
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| 60. |
List the advantages of recombinant insulin. |
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Answer» The advantages of recombinant insulin are (i) There is no need of animals to be sacrificed for the production of recombinant insulin. (ii) Recombinant insulin was not found allergic to patients, while the insulin from an animal source caused some patients to develop allergy or other types of reactions to the foreign protein. (iii) The cost of recombinant insulin is not very high. (Supply being many fold higher than demand). |
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| 61. |
Which bateria is used as biopesticide first on the commerical scale in the worldA. Bacillus thringginesisB. e coliC. pseudomonas aeruginosaD. agrobacterium tumefaciens |
| Answer» Correct Answer - A | |
| 62. |
What is meant by the term biopesticide? Name and explain the mode of action of a popular biopesticides. |
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Answer» Biopesticide is a pesticide which is (i) Not chemcial in nature (ii) More specific in action against the pest. (iii) Safer for environment than chemical pesticides. A popularly known bio-pesticide is Bt toxin. Which is produced by a bacterium called Bacillus thuringlensis. Bt toxin gene has been cloned from t his bacterium and expressed in plants. Bt toxin protein when ingested by the insect gets converted to its active form due to the alkaline pH of the gut. The activated toxin binds to the surface of midgut epithelial cells are create pores that causes swelling and lysis of the cell and eventually kills the insect. |
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| 63. |
Find out the pairs which are correctly matched `{:(a.,"Cyanobacteria",1.,"Biopesticide"),(b.,"Mycorrhiza",2.,"Solubilisation of phosphate"),(c.,"Bacillus thuringiensis",3.,"Cry protein"),(d.,"Single cell protein",4.,"Rhizobia"):}`A. I and IIB. II and IIIC. III and IVD. I and III |
| Answer» Correct Answer - D | |
| 64. |
Assertion Terrorists are trying to adopt bioweapons these days Reason Bioweapons ar liked by terrorist organisztions as these are costlyA. If both Assertion and Reason are true and the Reason is a correct explanation of the AssertionB. If both Assertion and Reason are ture but Reason is not a correct explanation of the AssertionC. If Assertion is true but the Reason is falseD. If both Assertion and Reason are false |
| Answer» Correct Answer - C | |
| 65. |
Name of the drug used in cancer treatment produced by using biotechnology:A. terramycinB. hghC. insulinD. tsh |
| Answer» Correct Answer - E | |
| 66. |
Hybridomas are produced by the fusion ofA. liver cells and myeloma cellsB. B - cells and myeloma cellsC. liver and ` beta ` - cellsD. leucocytes and myelome cells |
| Answer» Correct Answer - B | |
| 67. |
In protoplast fusion which chemical is usedA. DMSOB. liquid ` N _ 2 `C. pectinaseD. PEG |
| Answer» Correct Answer - D | |
| 68. |
Chemofusion and electrofusion are employed inA. eugenicsB. protoplast fusionC. cloningD. mutations |
| Answer» Correct Answer - B | |
| 69. |
Fill the blanks (a) single cell protein (SCP) provied a valuable …………rich supplement in ……………..diet (b) fruit softening in tomato is promoted by the enzyme ………………..which degrades pectin (C ) A soil bacterium ……………………produces a crystal (cry) protein (d)Ti plasmid is found in.......................... (E ) pentadipandra brazzeana a awest aftican plnant produces a protein called ......................which is approximately 2000 times as ................s sugar |
| Answer» (a) protein human (B) polygalacturonase, (c ) Bascillus thuringiensis , (D) A grobacterium tumefaciens , (E ) Barzzein sweet | |
| 70. |
Which one of the following is commonly used in transfer of foreign DNA into crop plants ?A. meloidogyne incognitaB. agrobactreium tumefaciensC. penicillium expansumD. trichoderma harizanum |
| Answer» Correct Answer - B | |
| 71. |
Assertion : Recognition site should be perfectly single and reponsive to commonly used restriction enzymes. Reason: In pNR 322 Alien DNA is ligated generally in the area of Bam-HI site of tetracycline resistance gene.A. If both Assertion and Reason are true and the Reason is a correct explanation of the AssertionB. If both Assertion and Reason are ture but Reason is not a correct explanation of the AssertionC. If Assertion is true but the Reason is falseD. If both Assertion and Reason are false |
| Answer» Correct Answer - A | |
| 72. |
Which one of the following is commonly used in transfer of foreign DNA into crop plants ?A. Meloidogyne inognitaB. Agrobacterium tumefaciensC. Penicillium expansumD. Trichoderma harzianum |
| Answer» Correct Answer - B | |
| 73. |
The introduction of t-DNA into plants involvesA. Infection of the plant by Agrobacterium tumefaciensB. altering the pH of soil, heat-shocking the plantsC. exposing the plants to cold for a frief periodD. allowing the plant roots to stand in water. |
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Answer» Correct Answer - A Agrobacterium tumefaciens, a pathogen of several dicot plants is able to deliver a piece of DNA known as tDNA to transform normal plant cells into a tumour and direct these tumour cells to produce the chemical required by the pathogen. |
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| 74. |
Which of the following Bt crops is being grown in India by the farmers ?A. MaizeB. CottonC. BrinjalD. Soyabean |
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Answer» Correct Answer - B In india Bt cotton is grown by farmers extensive. Bt toxin is poruced by a bacterium called bacillus thuringiensis (Bt). Examples of Bt crops are Bt cotton, Bt corn, rice, tomato, potato and soyabean etc. |
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| 75. |
Assertion Industrial fermentiations are money making ventures Reason They require a thorough reasech to discover high yilds fo produce at least possible expensesA. If both Assertion and Reason are true and the Reason is a correct explanation of the AssertionB. If both Assertion and Reason are ture but Reason is not a correct explanation of the AssertionC. If Assertion is true but the Reason is falseD. If both Assertion and Reason are false |
| Answer» Correct Answer - B | |
| 76. |
Which vector has employed for introducing nematode-specific genes in infected tobacco plants? |
| Answer» Agrobacterium vector | |
| 77. |
The genetic defect-adenosine deaminase (ADA) deficiency may be cured permanently byA. Administering adenosine deaminase activatorsB. Introducing bone marrow cells producing ADA mto cells at early embryonic stagesC. Enzyme replacement therapy.D. Periodic infusion of genetically engineered lymphocytes having functional ADA cDNA |
| Answer» Correct Answer - B | |
| 78. |
Which kind of therapy was given in 1990 to a four year old girl with adenosine deaminase (ADA) deficiency?A. gene threapyB. chmotherapyC. immunotherapyD. radiation therapy |
| Answer» Correct Answer - A | |
| 79. |
Which vector is used introduce a functional ADA cDNA into the lymphocytes? |
| Answer» Correct Answer - Retroviral vector | |
| 80. |
ADA is an enzyme which is deficient in a ge- netic disorder SCIO. What is the full form of ADA?A. Adenosine deoxy aminaseB. Adenosine deaminaseC. Aspartate deaminaseD. Arginine deaminase |
| Answer» Correct Answer - b | |
| 81. |
Injecting functional adenosine deaminase into a patients (lacking ADA gene) blood cells may be considered asA. Gene therapyB. Enzyme replacement therapyC. Both (1) & (2)D. Genetic engineering |
| Answer» Correct Answer - B | |
| 82. |
A functional ADA cDNA can be introduced into cells of the patients receiving gene therapy by using vector constituted byA. E. coliB. reovirusC. retrovirusD. agrobacterium |
| Answer» Correct Answer - C | |
| 83. |
Which vector is used introduce a functional ADA cDNA into the lymphocytes?A. AgrobacteriumB. ReovirusC. E.coliD. Retrovirus |
| Answer» Correct Answer - D | |
| 84. |
Which of these can be a defence against bioweapon ?A. Protective shelterB. ImmunisationC. Gas maskD. All of these |
| Answer» Correct Answer - D | |
| 85. |
Biological weapons convention and treaty given inA. 1972B. 1942C. 1932D. 1955 |
| Answer» Correct Answer - A | |
| 86. |
What are the various advantages of using genetically modified plants to increase the overall yield of the crop? |
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Answer» Genetically modified plants have been useful in ma ny ways as follows. (i) These plants are more tolerant to abiotic stresses (cold,drought, salt, heat) (ii) They have reduced the relance on chemical pesticides (pest-resistant crops). (iii) They helped to reduce post harvest losses. (iv) They increases the efficiency of mineral usage by plants this prevents early exhaustion of fertility of soil. (v) Enhanced nutritional value of food e.g., vitamin-A enriched rice. In addition to these uses. GM plants have been used to create tailor-made plants to supply alternative rescurces to industries, in the form of starches, fuels and pharmaceuticals. |
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| 87. |
GEAC stands forA. Genome En gineering Action CommitteeB. Ground Environment Action committeeC. Genetic En gineering Approval CommitteeD. Genetic and Enviroment Approval Committee. |
| Answer» GEAC stands for Genetic Engineering Approval Committee. The indian government has set up this organisation to make decisions regarding the validity of GM research and the safety of introducing GM-organisms for public services. | |
| 88. |
What is GEAC and what are its objectives? |
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Answer» GEAC (Genetic Engineering Approval Committee) is an inidan Government organisation. Its objectives include. (i) Examine the validity of Genetic Modification (GM) of organism research (ii) Inspect the safety of introducing GMO for public services. |
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| 89. |
What does GEAC stand for ? |
| Answer» Genetic Engineering Approval Committee. | |
| 90. |
Transgenic animals have been created that can produce `alpha-1`-antitrypsin in their milk. This protein is used to treat which disorder? |
| Answer» Correct Answer - Emphysema | |
| 91. |
Bacillus thuringiensis (Bt) strains have been used for designing novelA. biofertilisersB. bio - metallurgical techniquesC. bio - mineralisation processD. bio - insecticidal plants |
| Answer» Correct Answer - D | |
| 92. |
Differentiate between diagnostics and therapeutics, Give one example for each category. |
| Answer» A diagnostic techinuqe helps us to identify a disease e.g., ELISA for HIV. A therapeutic agent on the other hand helps in the treatment of a disease e.g., antibiotics for bacterial infections. | |
| 93. |
Who was the first patient to b e treated with gene therapy? Why was the given treatment recurrent in nature? |
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Answer» Gene therepy is a collection of methods that allows the correction of gene defects diagnosed in a child or embryo correction of a genetic defect involves the dlivery of a normal gene into the individual or embryo to take over the function of and com pensate for the non-functional gene. The first clinical gene therepy was given in 1990 to a 4 yrs old girl with Adenosine Deaminase (ADA) deficiency. ADA deficiency is caused due to the deletion of gene for Adenosine Deaminase. it can be cured by bone marrow trasnplantation or by enzyme replacement therapy. in both the approaches. it is not completelyy curable. It may recurrent in nature because in the process of gen e therapy, lymphocytes used are found to be mortal in nature and the patient requires periodic infusion of such genetically engineered lymphocytes. For permanent cure, gene isolated from the bone marrow. cells producing ADA is introduced into the cells at early embryonic statges. |
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| 94. |
A probe which is a molecule used to locate specific sequence in a mixture of DNA or RNA molecules could beA. a single standard RNAB. a single strandard DNAC. either RNA or DNAD. can be ssDNA but not ssRNA |
| Answer» A probe is a single stranded DNA or RNA tagged with a radioactive molecule it is used to defect the complementary sequences by hybridisation techniques. | |
| 95. |
A probe is used in which stage of genetic engineering?A. Cleaving DNAB. Recombining DNAC. CloningD. Screening |
| Answer» Correct Answer - D | |
| 96. |
Why is it that the line of treatment for a genetic disease is different from infectious diseases? |
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Answer» The line of treatment for a genetic disease is different from infectious diseases because genetic disease cannot be treated with any medication only the signs and symptoms can be taken care of. The only wasy to treat them is by the manipulation of genes to correct or replace the faulty genes. On the other hand, infectious diseases are caused by pathogens and therefore, can be treated by substances that kill the pathogen or hamper its growth. |
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| 97. |
Assertion: Yeasts should not be used in brewing and baking industries. Reason. They produce several harmpul products during brewing and baking.A. If both Assertion and Reason are true and the Reason is a correct explanation of the AssertionB. If both Assertion and Reason are ture but Reason is not a correct explanation of the AssertionC. If Assertion is true but the Reason is falseD. If both Assertion and Reason are false |
| Answer» Correct Answer - D | |
| 98. |
Immobilished enzymes are obtained by ……method.A. entrapmentB. ionic bindingC. cross linking with glutaraldehydeD. All of the above |
| Answer» Correct Answer - D | |
| 99. |
Name the two vitamins produced by microbial fermentation. |
| Answer» Vitamin `B_(12)` (cobalamine) and vitamin `B_(2)` (Riboflavin) | |
| 100. |
What are hybridomas |
| Answer» Fusion o fmyelocma cells and antibodyt producing cells results in the formation of fused cells called hybridomas | |