InterviewSolution
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InterviewSolution
This section includes InterviewSolutions, each offering curated multiple-choice questions to sharpen your knowledge and support exam preparation. Choose a topic below to get started.
| 51. |
Process used for amplification ro multiplication of DNA in DNA fingerprinting isA. polymerase chain reactionB. southern blottingC. northern blottingD. None of these |
| Answer» Correct Answer - A | |
| 52. |
Which of the following statements are correct for the enzyme Taq polymerase ? (i) It remains active during the hight temperature induced denaturation of dsDNA. (ii) It required primers for carrying out the process of polymerisation. It synthesises the RNA region between the primers, using dNTPs and `Mg^(2+)`.A. (i) and (ii)B. (ii) and (iii)C. (i),(ii) and (iii)D. None of these |
| Answer» Correct Answer - A | |
| 53. |
Match column I (enzyme) with column II (characteristic/activity) and select the correct answer from the given codes, `{:(Column I,Column II),(A."Taq DNA polymerase",(i)"Cleaves the ends of linear DNA"),(B."Exonuclease",(ii)"Breakdown of ungal cell wall"),(C."Protease",(iii)"Stable above" 90^(@)C),(D."Chitinase",(iv)"Made only by eukaryotic cells"),(,(v)"Degradation of proteins"):}`A. `A-(iii),B-(iv),C-(i),D-(ii)`B. `A-(iv),B-(iii),C-(i),D-(ii)`C. `A-(ii),B-(i),C-(v),D-(iii)`D. `A-(iii),B-(i),C-(v),D-(ii)` |
| Answer» Correct Answer - D | |
| 54. |
The taq polymerase enzyme is obtained fromA. Pseudomonas putidaB. Thermus aquaticusC. Thiobacillus ferroxidansD. Bacillus subtillis |
| Answer» Correct Answer - B | |
| 55. |
PCR technique is best forA. DNA synthesisB. Protein amplificationC. DNA amplificationD. DNA ligation |
| Answer» Correct Answer - C | |
| 56. |
In a polymerase chain reaction after the denaturation step why the mixture needs to cool down to a lower temperature ?A. To permit specific annealing of the primersB. To give a halt to the reaction mixtureC. To increase the activity of enzyme Taq polymeraseD. To obtain the multiple copies of the DNA |
| Answer» Correct Answer - A | |
| 57. |
Which of the following are not required while performing a transformation experiment ?A. Insert DNAB. Ice cold calcium chlorideC. `90^(@)`D. Bacterial cells |
| Answer» Correct Answer - C | |
| 58. |
Which of the following methods gave birth to idea of gene immunization ?A. MicroinjectionB. Direct DNA injectionC. BiolisticD. Both (1) & (3) |
| Answer» Correct Answer - B | |
| 59. |
Which of the following is not an application of PCR ?A. ELISAB. Diagnosis of pathogensC. DNA fingerprintingD. In palaeontology |
| Answer» Correct Answer - A | |
| 60. |
Name the organism which is the source of heat -stable PCR enzymeA. Thermophilus aquaticusB. Haemophillus influenzaeC. Thermo proteusD. Bacillus thuringiensis |
| Answer» Correct Answer - A | |
| 61. |
Which of the following is not correct for a successful PCR ?A. Thermostable DNA polymeraseB. Nanogram of DNA templateC. Primer with exposed 5-OH group onlyD. Deoxyribonucleoxide triphosphate |
| Answer» Correct Answer - C | |
| 62. |
PCR is used forA. Reverse transcribing RNA into DNAB. Digesting DNAC. Amplifying DNAD. Amplifying proteins and separating DNA |
| Answer» Correct Answer - C | |
| 63. |
Method in which recombinant DNA is directly injected into the nucleus of animal cell by using microneedles is calledA. Gene Gun methodB. Biolistic methodC. Microinjection methodD. Direct DNA injection method |
| Answer» Correct Answer - C | |
| 64. |
Method in which recombinant DNA is directly injected into the nucleus of animal cell by using microneedles is calledA. Gene Gun methodB. Biolistic methodC. Microinjection methodD. Indirect method |
| Answer» Correct Answer - C | |
| 65. |
The figure below is the diagrammatic representation of the E.coli vector pBr 322. which one of the given options correctly identifies its certain component (s) A. `amp^(R)` ,`tet^(R)-antibiotic ` resistance genesB. Ori-original restriction enzymeC. Rop-reduced osmotic pressureD. Hind III , EcoRI-selectable markers |
| Answer» Correct Answer - A | |
| 66. |
The transfer of genetic material from one bacterium to another through the mediation of a vector like virus is termed asA. transductionB. conjugationC. transformationD. translation |
| Answer» Correct Answer - A | |
| 67. |
Select the correct option to fill up balnks. (i) ________is a natural polymer extracted from_____. (ii) The DNA fragments purified by gel electrophoresis are used in constructing______by joining them with_______. (iii) The ligation of alien DNA is carried out at a________. present in one of the two________in a plasmid vector. (iv)_________enzyme remains active during the high temperature induced denaturation of ds DNA (v) DNA fragments are resolved according to their_________ through ______in agarose gel electrophoresis.A. (i) Agarose, sea weeds (ii) recombinant DNA, cloning vector (iii) restriction site, antibiotic resistance genes (iv) Taq polymerase (v) size, sieving effectB. (i) Agarose, sea weeds (ii) Restriction site, antibiotic resistance genes (iii) recombinant DNA, cloning vector (iv) Taq polymerase (v) size, sieving effectC. (i) Agarose, sea weeds (ii) restriction site, antibiotic resistance genes (iii) recombinant DNA, cloning vector (iv) Taq polymerease (v) size, sieving effectD. (i) size, sieving effect (ii) agarose, eas weeds (iii) recombinant DNA cloning vector (iv) Taq polymerase (v) restriction site, antibiotic resistance genes |
| Answer» Correct Answer - A | |
| 68. |
Rising of dough is due isA. multiplication of yeastB. production of `CO_(2)`C. emulsificationD. hydrolysis of wheat flour strach into sugars. |
| Answer» Correct Answer - B | |
| 69. |
Plasmids are important in biotechnology because they contianA. Recognition sites on recombinant DNA strandsB. Provirus incorporated into the host DNAC. A vehicle for insertion of recombinant DNA into bacteriaD. Surface for respiratory process in bacteria |
| Answer» Correct Answer - C | |
| 70. |
All the following statements about Stanley Cohen and Herbert Boyer are correct but one is wrong. Which one is wrongA. They discovered recombinant DNA (r-DNA) technology which marked the birth of modern biotechnologyB. They first produced. Healthyl sheep clone, a Finn Dorst lamb, Dolly from the differentaited adult mammary cells.C. They invented genetic engineering by combining a piece of foreign DNA containing a gene from a bacterium with a bacterial (E coli) plasmid using the enzyme restriction endonucleaseD. They isolated the antibiotic resistance gene by cutting out a piece of DNA from a plasmid which was responsible for conferring antibiotic resistance . |
| Answer» Correct Answer - B | |
| 71. |
Which of the following restriction enzymes produces blunt ends ?A. Sal IB. Eco RVC. Xho ID. Hind III |
| Answer» Correct Answer - B | |
| 72. |
Restriction endonucleases are enzymes which:A. Remove nucleotides from the ends to the DNA moleculsB. Make cuts at specific positions within the DNA moleculesC. Recognize a specific nucleotide sequence for binding of DNA ligasD. Restrict the action of the enzyme DNA polymerase |
| Answer» Correct Answer - B | |
| 73. |
Each restriction endonuclease functions by inspecting the length of a DNA sequence . It cleaves __________A. Only the master strand to produc sticky endB. Sense strand of DNA to produce sticky endsC. Each of the two strands of the double helix at specific points in their sugar phosphate backbonesD. Messenger RNA to remove exons |
| Answer» Correct Answer - C | |
| 74. |
Gel electrophoresis separates DNA molecules according to their ______. |
| Answer» Correct Answer - Size | |
| 75. |
Which of the given statements is correct in the context of observing DNA separated by agarose gel electrophoresis ?A. DNA can be seen in visible lightB. DNA can be seen without staining in visible lightC. Ethidium bromide stained DNA can be seen in visible lightD. Ethidium bromide stained DNA can be seen under exposure to UV light |
| Answer» Correct Answer - D | |
| 76. |
A: Restriction enzymes belong to a larger class of enzymes called nucleases. R: Each restriction enzymes recognises sequence in the DNA.A. If both Assertion & Reason are true and the reason is the correct explanation of the assertionB. If both Assertion & Reason are true but the reason is not the correct explanation of the assertionC. If Assertion is true statement but Reason is falseD. If both Assertion and Reason are false statements |
| Answer» Correct Answer - B | |
| 77. |
A: Selection of recombinants due to inactivation of antibiotics is cumbersome procedure. R: It requires simultaneous plating on two plates having different antibiotics.A. If both Assertion & Reason are true and the reason is the correct explanation of the assertionB. If both Assertion & Reason are true but the reason is not the correct explanation of the assertionC. If Assertion is true statement but Reason is falseD. If both Assertion and Reason are false statements |
| Answer» Correct Answer - A | |
| 78. |
When of the following steps should be performed by a preson in order to visualise the bands of DNA fragments obtained from gel electrophoresis ?A. Exposure of DNA fragments to UV radiationsB. Staining with bromophenol blue followed by exposure to UV radiationsC. Staining with ethidium bromide followed by exposure to UV radiations.D. Person can see the bands without staining. |
| Answer» Correct Answer - C | |
| 79. |
The DNA fragments separated on an agarose gel can be visualised after staining withA. Bromophenol blueB. AcetocarmineC. Aniline blueD. Ethidium bromide |
| Answer» Correct Answer - D | |
| 80. |
A: During gel electrophoresis, the DNA fragments move towards the anode. R: DNA fragments are negatively charged molecules .A. If both Assertion & Reason are true and the reason is the correct explanation of the assertionB. If both Assertion & Reason are true but the reason is not the correct explanation of the assertionC. If Assertion is true statement but Reason is falseD. If both Assertion and Reason are false statements |
| Answer» Correct Answer - A | |
| 81. |
What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis ?A. The large the fragmetn size , the farther it movesB. The smaller the fragment size, the farther it movesC. Positively charged fragments move to farther endD. Negatively charged fragments do not move |
| Answer» Correct Answer - B | |
| 82. |
Which vector can clone only a small fragment of DNA ?A. Bacterial artificial chromosomeB. Yeast artificial chromosomeC. PlasmidD. Cosmid |
| Answer» Correct Answer - C | |
| 83. |
What is a clone ? |
| Answer» Clone is a group of cells with identical genotypes derived fro same mother cell. | |
| 84. |
What is the basis of selection in a plasmid-containing gene lac Z ? |
| Answer» Lac Z codes for enzyme `beta`-galactosidase which produces coloured product on acting on its substrate (chromogenic selection) | |
| 85. |
List two commonly used vectors. |
|
Answer» 1. Plasmids -pBR322 2. Bacteriophage -`lambda` |
|
| 86. |
Boliver and Rodriguez developedA. Shuttle vectorB. pBR322C. Puc19D. Both (1) & (2) |
| Answer» Correct Answer - B | |
| 87. |
Shuttle vectors are not exemplified byA. YepB. Ti plasmidC. BACD. Both (2) & (3) |
| Answer» Correct Answer - C | |
| 88. |
In genetic engineering, the antibiotics are usedA. To keep the cultures free of infectionB. As selectable mrarkersC. To select healthy vectorsD. As sequences from where replication starts |
| Answer» Correct Answer - B | |
| 89. |
Which vector is used to introduce genes into dicots ?A. ElectroporationB. ElectrophoresisC. Ti plasmid infectionD. Microinjection |
| Answer» Correct Answer - C | |
| 90. |
Addition of antibiotics in chemical engineering process helps inA. Mixing and aeration of mediaB. Maintaining anti-foaming conditionsC. Maintaining aseptic conditionsD. Maintainance of constant temperature and Ph |
| Answer» Correct Answer - C | |
| 91. |
The linking of antibiotic resistance gene with the plasmid vector became possible withA. DNA polymeraseB. ExonucleaseC. DNA ligaseD. Endonuclease |
| Answer» Correct Answer - C | |
| 92. |
Chemical knives of molecular biology areA. Restriction endonucleaseB. ExonucleaseC. Reverse transcriptaseD. Ligase |
| Answer» Correct Answer - A | |
| 93. |
Which of the following enzymes has been incorrectly matched with their function ?A. Ligase -Molecular glueB. Endonuclease -Chemical scalpelC. DNA polymerase-Joins nucleosidesD. RNA polymease-Joins nucleotides |
| Answer» Correct Answer - C | |
| 94. |
Fill up the blanks and select the correct option. (i) EcoRl cuts the DNA between bases_______only when the sequence______is present in the DNA duplex. (ii) Disruption of the cell membrances can be achieved by treating the bacterial cells, plant cells and fungal cells with enzymes respectively_______,________and ______. (iii) Since DNA has a_______charge, it moves towards the__________of the electrophoretic chamber.A. (i) G and A, GA A T TC (ii) endonuclease, cellulase, chitinase (ii) negative, anodeB. (i) G and A,G A AT TC(i) lysozyme, cellulase, chitinase (iii) positive, cathodeC. (i) G and A,GA AT C (ii) lysozyme, cellulase, chitinase (ii)negative, anodeD. (i) G and A, GA ATC (ii) lysozyme, cellulase, chitinase (iii) positive, cathode |
| Answer» Correct Answer - C | |
| 95. |
Restrictioni enzymes work under optimal conditions . Commnet. |
| Answer» RE requires `37^(@)C` , pH (variable) and buffered `H_(2)O` | |
| 96. |
A: Ethidium bromide helps in visualizing DNA in UV light only. R: This intercalating agent absorbs light in range 260-330 nm.A. If both Assertion & Reason are true and the reason is the correct explanation of the assertionB. If both Assertion & Reason are true but the reason is not the correct explanation of the assertionC. If Assertion is true statement but Reason is falseD. If both Assertion and Reason are false statements |
| Answer» Correct Answer - A | |
| 97. |
The first restriction enzyme to be isolated and characterised wasA. EcoRIB. BamHIC. Hind IIID. Hind II |
| Answer» Correct Answer - D | |
| 98. |
Match column I with column II with respect to the nomenclature of restriction enzyme EcoRl and select the correct answer from the given codes. `{:(Column I,Column II),(A.E,(i)1^(st)"in order of identification"),(B.co,(ii)"Name of genus"),(C.R,(iii)"Name of species"),(D.l,(iv)"Name of strain"):}`A. `A-(iii),B-(i),C-(ii),D-(iv)`B. `A-(ii),B-(i),C-(iii),D-(iv)`C. `A-(i),B-(ii),C-(iii),D-(iv)`D. `A-(ii),B-(iii),C-(iv),D-(i)` |
| Answer» Correct Answer - D | |
| 99. |
The source of the restriction enzyme Hindll isA. Escherichia coli `RY 13`B. Haemophilus influenzae RdC. Bacillus amyloliuefaciens `H`D. Streptomyces albus |
| Answer» Correct Answer - B | |
| 100. |
Give an example of disarmed viruses used as vector in animals . |
| Answer» Correct Answer - Retroviruses | |