InterviewSolution
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InterviewSolution
This section includes InterviewSolutions, each offering curated multiple-choice questions to sharpen your knowledge and support exam preparation. Choose a topic below to get started.
| 101. |
A: Pst I generates single stranded over hanging stretches in DNA after digestion that facilitate ligation. R: These short extensions can form phosphodiester bonds with their complementary counterparts.A. If both Assertion & Reason are true and the reason is the correct explanation of the assertionB. If both Assertion & Reason are true but the reason is not the correct explanation of the assertionC. If Assertion is true statement but Reason is falseD. If both Assertion and Reason are false statements |
| Answer» Correct Answer - C | |
| 102. |
cDNA isA. Formed by reverse transcriptaseB. Cloned DNAC. Circular DNAD. Recombinant DNA |
| Answer» Correct Answer - A | |
| 103. |
How long is the recognition sequence of the first restriction enzyme isolated ? |
| Answer» Correct Answer - Six base pairs | |
| 104. |
Downstreaming process in biotechnology refers toA. The process which includes separation and purification of the product , after the completion of the biosynthetic stageB. Large scale production of a product , by using bioreactorsC. The cells harbouring cloned genes of interest being grown on a small scaleD. The microbes which act upon the substrate are cultured and added into the fermenter |
| Answer» Correct Answer - A | |
| 105. |
A: Disarmed Ti plasmid can be utilised as a shuttle vector. R: It can survive in both prokaryotic and eukaryotic organisms.A. If both Assertion & Reason are true and the reason is the correct explanation of the assertionB. If both Assertion & Reason are true but the reason is not the correct explanation of the assertionC. If Assertion is true statement but Reason is falseD. If both Assertion and Reason are false statements |
| Answer» Correct Answer - A | |
| 106. |
How has Ti plasmid been disarmed ? |
| Answer» By removal of T-RNA region of plasmid that can transform normal plant cells into tumor cells | |
| 107. |
Discovery of which made genetic engineering possible ? |
| Answer» Restriction endonucleases | |
| 108. |
Two microbes found to be very useful in genetic engineering are :A. Escherichia coli and AgrobacteriumB. Vibrio cholerae and a tailed bacteriophageC. Diplococuus sp. And Pseudomonas sp.D. Crown gall bacterium and Caenorhabditis |
| Answer» Correct Answer - A | |
| 109. |
In RDT, the term vector refers toA. Plasmids that can transfer foreign DNA into a living cellB. Plasmids that can cut DNA at specific basesC. Plasmids that can join DNA at specific basesD. Plasmids that can degrade harmful protiens |
| Answer» Correct Answer - A | |
| 110. |
Although the Ti plasmid has revolutionised plant genetic engineering one limitation of its use is that itA. Cannot infect broad leaf plantsB. Cannot be used on fruits-bearing plantsC. Cannot be used on fruits-bearing plantsD. Does not infect cereal plants such as corn and rice |
| Answer» Correct Answer - D | |
| 111. |
Genetic engineering is possible becauseA. DNA can be cut at specific site by endonucleases like DNAaseB. Restriction endonucleases purified from bacteria can be used in vitroC. The phenomenon of transduction in bacteria is well understoodD. DNA can be seen by electron microsocpe |
| Answer» Correct Answer - B | |
| 112. |
Pure DNA precipated by addition of chilled ethanol can be removed from solution byA. ElutionB. Gel electrophoresisC. SpoolingD. PCR |
| Answer» Correct Answer - C | |
| 113. |
Bacterium commonly used in plant genetic engineering isA. E. coliB. AgrobacteriumC. MycobacteriumD. Rhizobium |
| Answer» Correct Answer - B | |
| 114. |
Genetic material of retroviruses isA. DNAB. RNAC. ProteinD. ssDNA |
| Answer» Correct Answer - B | |
| 115. |
Which of the following methods can be used for making the bacterial cell " competent" ?A. Treating with specific concentration of divalent cation `( Ca ^(+2) )`B. Treating with specific cocentration of monovalent cation ` (K^(+) )`C. Heat shockD. Both (1) & (3) |
| Answer» Correct Answer - D | |
| 116. |
Isolation of genetic material from fungal cells involves the use ofA. LysozymeB. CellulaseC. ChitinaseD. Polymerase |
| Answer» Correct Answer - C | |
| 117. |
While isolating DNA from bacteria, which of the following enzymes is not used ?A. LysozymeB. RibonucleaseC. ProteaseD. Deoxyribonuclease |
| Answer» Correct Answer - D | |
| 118. |
Restricito enzymes cut the strand of DNA a little away from the centre of the palindromic site, but between the same two bases on the opposite single stranded strand, these overhanging stretches formed on each strand are called __________A. Blunt endsB. Stickly endsC. Staggered endD. Both (2) & (3) |
| Answer» Correct Answer - D | |
| 119. |
Telomeric sequence, centromere , autonomously replicating sequence from yeast are characteristics features ofA. YACB. BacteriaC. RetrovirusD. All of these |
| Answer» Correct Answer - A | |
| 120. |
Which of the following is not a basis of difference between plasmid and chromosomal DNA ? a.Presence of histones b. Nature of histones c. Nature of nucleotide d. Linear form of genetic materialA. b & c onlyB. a & d onlyC. a , b , & d onlyD. a , b , c & d |
| Answer» Correct Answer - A | |
| 121. |
An antibiotic resistance gene in a vector usually helps in the selection ofA. Competent cellsB. Transformed cellsC. Recombinant cellsD. Both (2) & (3) |
| Answer» Correct Answer - D | |
| 122. |
Stirreer in stirred tank type bioreactor facilitiesA. Oxygen delivery from outside to insideB. Mixing and aerationC. Temperature controlD. Foam control |
| Answer» Correct Answer - B | |
| 123. |
A gene whose expression helps to identify transformed cell is known asA. Selectable markerB. VectorC. PlasmidsD. Structural gene |
| Answer» Correct Answer - A | |
| 124. |
In case of Bam HI , H representsA. GenusB. SpeciesC. Name of scientistD. Strain |
| Answer» Correct Answer - D | |
| 125. |
Which of the following represents an advantage of growing cells in continuous culture as compared to a shake flask ?A. Cells can be maintained at a constant physiological stateB. Most downstream and upstream processes are continuous in natureC. Continuous reactors do not need to be shut down and cleaned regularlyD. All of these |
| Answer» Correct Answer - D | |
| 126. |
Cells in continuous culture system are maintained inA. Stationary phaseB. Lag phaseC. Log phaseD. Either (1) or (3) |
| Answer» Correct Answer - C | |
| 127. |
A device in which large volume of living cells are cultured in order to get a specific product is calledA. PCRB. agitatorC. bioreactorD. assimilator |
| Answer» Correct Answer - C | |
| 128. |
Which of the following statements are correct with respect to a biorector ? (i) It can process large volumes of culture. (ii) It provides optimum temperature and pH. (iii) It is a completely automated tool. (iv) It is a compact thermal cycler.A. (i) and (ii)B. (i),(ii) and (iii)C. (iii) and (iv)D. (ii) and (iii) |
| Answer» Correct Answer - A | |