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51.	The temperature at which the shift in absorbance is half completed is termed as ___________(a) melting temperature(b) denaturation temperature(c) shifting temperature(d) absorbance temperatureI had been asked this question during a job interview.My doubt stems from Genome Structure topic in section Nature of Genome of Cell Biology
Answer» The correct option is (a) melting temperature To elaborate: When the DNA SEPARATES into two of its constituent strands. The hydrophobic interactions that result from base stacking are GREATLY reduced, which CHANGES the electronic nature of the bases and INCREASES their absorbance of ultraviolet radiation. The temperature at which the shift in absorbance is half completed is termed as the melting temperature Tm.

52.	Who was the first person to suggest that genes were capable of moving around the genome?(a) James Watson(b) Barbara McClintock(c) Louis Pasteur(d) Gregor MendelThe question was posed to me in an international level competition.My question is taken from Genome Stability topic in chapter Nature of Genome of Cell Biology
Answer» Right option is (B) Barbara McClintock Best explanation: Barbara McClintock was the first person to suggest that genes were CAPABLE of moving around the genome. She was a geneticist WORKING with maize at the COLD Spring Harbor Laboratories in New York.

53.	For three different samples of DNA with same concentration, the rate of annealing will depend on __________(a) pH(b) absorbance(c) temperature(d) genome sizeThis question was addressed to me in exam.I would like to ask this question from Genome Structure topic in portion Nature of Genome of Cell Biology
Answer» The CORRECT choice is (d) genome SIZE For explanation I would SAY: The rate of ANNEALING will depend upon the size of genome. SMALLER the genome, greater will be the rate of annealing.

54.	Proteins can interact with a DNA molecule by ____________________(a) fitting into the grooves(b) interacting with the base pairs(c) hydrophilic interactions(d) covalent linkagesThis question was posed to me in an international level competition.My question is taken from Gene Chemical Nature in section Nature of Genome of Cell Biology
Answer» The correct choice is (a) fitting into the grooves The explanation is: Proteins have domains that FIT into major and minor grooves of the DNA molecule. A PROTEIN interacting with the DNA in this way can read the SEQUENCE of NUCLEOTIDES without having to separate the strands.