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1.	The element, required for the activity of nitrogen-fixing microbes, is?(a) Cobalt only (b) Molybdenum only (c) Iron only (d) All the above
Answer» The answer is (d) All the above

2.	The symbiotic nitrogen-fixing bacterium is – (a) Nitrosomonas (b) Nitrobacter (c) Rhizobium (d) All the above
Answer» The answer is (c) Rhizobium

3.	What is leghaemoglobin?
Answer» Leghaemoglobin is a pink or reddish coloured protein pigment found in the functional nodules of leguminous plants. This pigment absorbs O₂ and protects the nitrogenase enzyme against inactivation due to the presence of O₂

4.	In blue-green algae which specialized cell performs nitrogen fixation?
Answer» The answer is Heterocyst

5.	What do you understand by symbiotic nitrogen fixation?
Answer» When nitrogen fixation is performed by a mutualistic relation established between a microbe and plant, the process is called symbiotic N₂ fixation. Here the two alone independently can not carry out the process and N₂ fixation has an outcome of the interaction of the microbe and the plant. Example: Root nodule of leguminous plants containing Rhizobium bacteria. Anabaena alga present in the leaves of Azolla (A pteridophyte).

6.	Why plants can’t use nitrogen directly in spite it’s presence in the atmosphere up to 78% by volume?
Answer» A plant can not use atmospheric nitrogen directly because it is present in free gaseous form in the atmosphere whereas plants are capable of absorbing in the form of nitrogen compounds only from the soil. In soil, nitrogen is found in the form of organic and inorganic compounds and is absorbed by the roots.