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51.	Which product formed in the isomerization of mannose 6-phosphate?(a) Fructose 6-phosphate(b) Glucose 6-phosphate(c) 2-phosphoglycerate(d) 3-phosphoglycerateThis question was posed to me during an interview.This key question is from Advanced Questions and Answers in chapter Regulation of Gene Expression and Miscellaneous of Biochemistry
Answer» The CORRECT OPTION is (a) Fructose 6-phosphate The explanation: MANNOSE 6-phosphate is isomerized by phosphomannose ISOMERASE to yield fructose 6-phosphate.

52.	Tertiary conformation of proteins is maintained by 3 types of bonds namely ionic, hydrogen and ____________(a) Sulfide(b) Disulfide(c) Covalent(d) PeptideThe question was posed to me during an internship interview.My enquiry is from Tricky Questions and Answers in chapter Regulation of Gene Expression and Miscellaneous of Biochemistry
Answer» Right option is (b) DISULFIDE To elaborate: Ionic INTERACTIONS, HYDROGEN and disulfide LINKAGES stabilizes the tertiary structure of a protein.

53.	1M NaCl and 1M HCl are present in an aqueous solution. What is the solution?(a) Not a buffer solution with pH < 7(b) Not a buffer solution with pH > 7(c) A buffer solution with pH < 7(d) A buffer solution with pH > 7I have been asked this question at a job interview.My doubt stems from Tricky Questions and Answers topic in division Regulation of Gene Expression and Miscellaneous of Biochemistry
Answer» Right CHOICE is (a) Not a BUFFER solution with pH < 7 The best explanation: It is a neutral solution and its pH = 7.

54.	When DNA segment is in one orientation ____________(a) The gene for FljB and gene encoding FljA are expressed(b) The gene for FliC are expressed(c) The gene for FljB and gene encoding FljA are not expressed(d) The gene for FljB and gene encoding FljC are not expressedThe question was posed to me during an interview.Asked question is from Regulation of Gene Expression in Prokaryotes in chapter Regulation of Gene Expression and Miscellaneous of Biochemistry
Answer» The correct CHOICE is (a) The gene for FljB and gene ENCODING FljA are EXPRESSED Explanation: When DNA SEGMENT is in one orientation, the gene for FljB and gene encoding FljA are expressed; the repressor SHUTS down expression of the gene for FliC.

55.	CAP is ____________(a) Catabolite gene activator protein(b) Catabolite gene repressor protein(c) Anabolite gene activator protein(d) Anabolite gene repressor proteinI have been asked this question by my college professor while I was bunking the class.My question is from Regulation of Gene Expression in Prokaryotes in portion Regulation of Gene Expression and Miscellaneous of Biochemistry
Answer» The correct option is (a) Catabolite gene ACTIVATOR PROTEIN Best explanation: CAMP receptor protein or CRP is sometimes CALLED CAP, catabolite gene activator protein.

56.	The mechanism by which CBP activates transcription is?(a) CBP has DNA methyl transferase activity(b) CBP has histone acetyl transferase activity(c) CBP interacts with the basal transcription complex(d) CBP interacts with the basal transcription complex and has histone acetyl transferase activityThe question was asked by my school teacher while I was bunking the class.Query is from Regulation of Gene Expression in Prokaryotes-1 topic in chapter Regulation of Gene Expression and Miscellaneous of Biochemistry
Answer» Right CHOICE is (c) CBP INTERACTS with the basal TRANSCRIPTION COMPLEX To ELABORATE: CBP interacts with the basal transcription complex and activates transcription.

57.	Which of the following statements regarding the regulation of trp operon expression by attenuation is correct?(a) Rapid translation of the leader peptide prevents completion of mRNA transcript(b) Rapid translation of the leader peptide allows completion of mRNA transcript(c) The leader peptide sequence encodes enzymes required for tryptophan synthesis(d) The leader peptide sequence contains no tryptophan residuesI got this question in a job interview.Question is taken from Regulation of Gene Expression in Prokaryotes-1 topic in portion Regulation of Gene Expression and Miscellaneous of Biochemistry
Answer» The correct choice is (a) Rapid TRANSLATION of the leader PEPTIDE PREVENTS completion of mRNA transcript The EXPLANATION: Attenuation results in PREMATURE termination of transcription.

58.	Which of the following bacterial operon is not controlled by attenuation?(a) Arabinose(b) Tryptophan(c) Leucine(d) HistidineThe question was asked during an interview for a job.The above asked question is from Regulation of Gene Expression in Prokaryotes-1 in chapter Regulation of Gene Expression and Miscellaneous of Biochemistry
Answer» The CORRECT CHOICE is (a) ARABINOSE For EXPLANATION: Arabinose is a CARBOHYDRATE.

59.	Which of the following are the structural motifs that mediate protein-protein interactions?(a) Leucine zipper and basic helix-loop-helix(b) Homeodomain(c) Zinc finger(d) Helix-turn-helixThe question was asked in a job interview.Question is from Principles of Gene Regulation in section Regulation of Gene Expression and Miscellaneous of Biochemistry
Answer» The correct choice is (a) Leucine zipper and basic helix-loop-helix The explanation: REGULATORY proteins contain DOMAINS not only for DNA binding but also for protein-protein interactions. Homeodomain, ZINC FINGER and helix-turn-helix are DNA-binding domains.

60.	The lac repressor has which of the following DNA-binding motif?(a) Helix-turn-helix(b) Zinc finger(c) Homeodomain(d) Leucine zipperI got this question in an international level competition.I would like to ask this question from Principles of Gene Regulation in section Regulation of Gene Expression and Miscellaneous of Biochemistry
Answer» The CORRECT answer is (a) Helix-turn-helix To EXPLAIN I would SAY: The LAC REPRESSOR has helix-turn-helix DNA-binding motif.