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101.

Which of the following can be used for selective precipitation of proteins?(a) alcohol(b) phenol(c) ammonium sulfate(d) sodium acetateThe question was posed to me by my school principal while I was bunking the class.This is a very interesting question from Protein Techniques in division Techniques in Cell Biology of Cell Biology

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Correct CHOICE is (c) ammonium sulfate

Easiest explanation: Ammonium sulfate is COMMONLY USED for selective PRECIPITATION of proteins. It is highly soluble in water and has high ionic strength. Purification is achieved by gradually adding the salt solution to crude protein EXTRACT.
Discussion
102.

Which of the following gives a three dimensional quality of the image?(a) Differential interference contrast(b) Bright-field microscope(c) Gas chromatography(d) SpectrophotometryThis question was addressed to me during an interview.This intriguing question originated from Techniques in portion Techniques in Cell Biology of Cell Biology

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Right choice is (a) Differential interference CONTRAST

Easiest explanation: The differential interference contrast (DIC) also termed as Nomarski interference delivers a three-dimensional quality of the image. The phase-contrast MICROSCOPE is another TYPE of interference microscope.
Discussion
103.

The biological materials have little intrinsic capability to ____________________(a) scatter electrons(b) stain(c) remain viable(d) be capturedThe question was posed to me by my school principal while I was bunking the class.This interesting question is from Techniques topic in portion Techniques in Cell Biology of Cell Biology

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The correct ANSWER is (a) scatter electrons

Easy explanation: The insoluble materials of CELLS contain ATOMS of low atomic number such as carbon, hydrogen, oxygen and nitrogen. The biological materials therefore have very little intrinsic capability of SCATTERING the electrons.
Discussion
104.

cDNA libraries are produced from ______________________(a) ribonucleic acids(b) messenger RNAs(c) transfer RNAs(d) ribosomal RNAsI had been asked this question by my school principal while I was bunking the class.This key question is from Techniques in chapter Techniques in Cell Biology of Cell Biology

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Right OPTION is (b) messenger RNAs

The explanation is: Complementary DNA (cDNA) LIBRARIES are produced from the messenger RNA molecules and REPRESENT the type of GENES residing in a particular type of cell. The other type of DNA libraries is the genomic library.
Discussion
105.

Which of the following is used for the preparation of a section specimen?(a) phenol(b) isoamyl alcohol(c) fixative(d) lubricativeThis question was posed to me during a job interview.This intriguing question originated from Techniques in portion Techniques in Cell Biology of Cell Biology

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The correct answer is (c) fixative

The best explanation: There are two TYPES of samples that can be used in the LIGHT MICROSCOPY – whole mount and sections. A section can be a THIN slice of an organelle that is not VIABLE but retains the morphology of the living structure.
Discussion
106.

When was the nucleotide sequence of a viral genome first elucidated?(a) 1977(b) 1988(c) 1999(d) 2002I had been asked this question by my school principal while I was bunking the class.This interesting question is from Techniques in portion Techniques in Cell Biology of Cell Biology

Answer» RIGHT choice is (a) 1977

Explanation: The first nucleotide SEQUENCE of an ENTIRE viral genome X174 was SEQUENCED in vitro in the year 1977. The nucleotide sequence CONTAINED 5375 nucleotides. Frederick Sanger was the first person to sequence a polypeptide.
Discussion
107.

Density gradient centrifugation is a type of isopycnic centrifugation.(a) True(b) FalseThe question was posed to me in homework.Question is taken from Techniques in portion Techniques in Cell Biology of Cell Biology

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The correct OPTION is (a) True

Easy explanation: Isopycnic CENTRIFUGATION SOLELY relies on density for separation. In density gradient centrifugation too the CONTENTS are separated with the help of an ADDITIVE with intermediate density of the particles that are being separated. It is therefore a form of Isopycnic separation.
Discussion
108.

In the secondary culture, cells are obtained from _______________________(a) primary culture(b) the organism(c) organ culture(d) phenotypic cultureI have been asked this question in my homework.My doubt is from Techniques topic in portion Techniques in Cell Biology of Cell Biology

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Correct choice is (a) primary CULTURE

Easiest explanation: The culture that is DONE after the primary culture is KNOWN as the SECONDARY culture (the process termed passaging or subculturing). The primary culture is obtained from the organism itself.
Discussion
109.

DNA libraries are collection of ______________________(a) ribonucleic acid(b) cloned DNA fragments(c) bacteriophages(d) viral particlesThis question was addressed to me in an interview.This intriguing question comes from Techniques in section Techniques in Cell Biology of Cell Biology

Answer» RIGHT answer is (B) cloned DNA fragments

The explanation is: DNA libraries are a collection of cloned DNA fragments; there are two types of DNA libraries NAMELY genomic libraries and complementary-DNA libraries. The libraries are instrumental for GENETIC researchers.
Discussion
110.

Polymerase chain reaction (PCR) was invented by ______________________(a) Kary Mullis(b) James Watson(c) John Hopkins(d) Hargobind KhoranaThe question was posed to me in semester exam.This question is from Techniques in section Techniques in Cell Biology of Cell Biology

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The correct option is (a) Kary Mullis

The best I can explain: Polymerase chain reaction (PCR) was CONCEIVED in 1983 by Kary Mullis of Cetus Corporation. This technique is widely used for the AMPLIFICATION of DNA SEQUENCES without the need for bacterial cells.
Discussion
111.

Which of the following is a commonly used label in blotting techniques?(a) vimentin(b) biotin(c) avidin(d) streptomycinI have been asked this question during an interview.I want to ask this question from Nucleic-acid Techniques in section Techniques in Cell Biology of Cell Biology

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Discussion
112.

One Svedberg unit is equivalent to 10^-13 ______________(a) hours(b) minutes(c) seconds(d) milli-secondsThe question was posed to me in an interview.Query is from Nucleic-acid Techniques in section Techniques in Cell Biology of Cell Biology

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Correct answer is (c) seconds

Explanation: ONE Svedberg UNIT is equivalent to a sedimentation coefficient of 10^-13 seconds. Every particle moves with its SPECIFIC sedimentation VELOCITY in RESPONSE to the centrifugal force.
Discussion
113.

Phenol is an active protein denaturant.(a) True(b) FalseThis question was posed to me in homework.I need to ask this question from Nucleic-acid Techniques topic in section Techniques in Cell Biology of Cell Biology

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Discussion
114.

Which of the following uses non-compressible matrix and high pressure?(a) HPLC(b) GC-MS(c) LC-MS(d) MS-MSThis question was posed to me by my college director while I was bunking the class.I want to ask this question from Protein Techniques topic in section Techniques in Cell Biology of Cell Biology

Answer» RIGHT ANSWER is (a) HPLC

Easy explanation: HIGH performance liquid chromatography (HPLC) uses LONG, narrow columns in while the mobile phase is forced through the non-compressible stationary phase under high pressure.
Discussion
115.

Liquid scintillation spectrometry is a method of detecting ________________________(a) X-rays(b) α-emitters(c) β-emitters(d) Gamma-raysI have been asked this question in class test.I want to ask this question from Techniques topic in section Techniques in Cell Biology of Cell Biology

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Correct answer is (c) β-emitters

The best I can explain: LIQUID scintillation spectrometry is a method of detecting the beta-emitters during disintegration of an atom. The technique is BASED on certain compounds termed phosphors that some of the ENERGY of emitted particles and RELEASE it in the form of light.
Discussion
116.

Which of the following component of TEM focuses the beam of electrons on the sample?(a) ocular lens(b) condenser lens(c) stage(d) columnThe question was asked in final exam.My question comes from Techniques in section Techniques in Cell Biology of Cell Biology

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Right option is (b) CONDENSER lens

Easiest explanation: The condenser lens focuses the electron beam on to the SPECIMEN, in case of transmission electron microscope. The specimen is supported on the GRID holder and placed INSIDE the COLUMN.
Discussion