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Which of the following can be used for selective precipitation of proteins?(a) alcohol(b) phenol(c) ammonium sulfate(d) sodium acetateThe question was posed to me by my school principal while I was bunking the class.This is a very interesting question from Protein Techniques in division Techniques in Cell Biology of Cell Biology

Answer»

Correct CHOICE is (c) ammonium sulfate

Easiest explanation: Ammonium sulfate is COMMONLY USED for selective PRECIPITATION of proteins. It is highly soluble in water and has high ionic strength. Purification is achieved by gradually adding the salt solution to crude protein EXTRACT.



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