Which is the main ingredient in the sample preparation of mass spectrometry?(a) papain(b) pepsin(c) vinculin(d) trypsinI had been asked this question in semester exam.The doubt is from Techniques topic in section Techniques in Cell Biology of Cell Biology

Which is the main ingredient in the sample preparation of mass spectro

1.	Which is the main ingredient in the sample preparation of mass spectrometry?(a) papain(b) pepsin(c) vinculin(d) trypsinI had been asked this question in semester exam.The doubt is from Techniques topic in section Techniques in Cell Biology of Cell Biology
Answer» The correct answer is (d) trypsin Easiest EXPLANATION: Trypsin is used in a mass spectrometric ANALYSIS to digest proteins. After protein digestion, there is a fractionation step USING the LIQUID chromatographic procedures. After that the samples are ionized and made gaseous.

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