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In which of the following techniques, the DNA is labeled using complementary sequences and then analyzed using autoradiography?(a) Western blotting(b) Southern blotting(c) Isopycnic centrifugation(d) SpectrophotometryI got this question in exam.My query is from Nucleic-acid Techniques topic in section Techniques in Cell Biology of Cell Biology

Answer»

Right choice is (b) Southern BLOTTING

Best explanation: Southern blotting is the technique where nucleic ACIDS are FIRST TRANSFERRED from gel to a nitrocellulose membrane. The membrane is then incubated in a solution of probes (complementary sequences) and then the bound sequences analyzed USING autoradiography.



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