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A functional ADA cDNA is introduced into the patient’s lymphocytes using _______ vector.(a) jumping(b) retroviral(c) infectious(d) bacterialThis question was posed to me during an online interview.My doubt is from Biotechnological Applications in Medicine: Gene Therapy topic in section Biotechnology and its Applications of Biology – Class 12

Answer»

Right choice is (b) retroviral

To explain I would say: In GENE therapy, a functional ADA cDNA is introduced into the patient’s LYMPHOCYTES using a retroviral vector. Retroviruses are used because they have an enzyme reverse TRANSCRIPTASE. This enzyme converts single-stranded RNA into double-stranded DNA and this DNA then further integrates within the GENOME without disturbing its stability.


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