Related InterviewSolutions

• How is copy number of the plasmid vector related to yield of recombinant protein?
• Would you choose an exonuclease, while producing a recombinant DNA molecule?
• Both a wire maker and a molecular biologist who had developed a recombinant vaccine claim to be biotechnologists. Who in your opnion is correct?
• Restriction enzymes that are used in the construction of recombinant DNA are endonucleases which cut the DNA at specific recognition sequence? What would be the disadvantage if they do not cut the DNA at specific recogniion sequence?
• A recombinant DNA molecule was created by ligating a gene to a plasmid vector. By mistake. An exonuclease was added to the tube containing the recombinant DNA. How does this affect the next step in the experiment, i.e., bacterial trasformation?
• What modification is done on the Ti-plasmid of Agrobacterium tumefaciens to convert it into cloning vector?
• An antibiotic resistance gene in a vector usually helps in the selection ofA. competent cellsB. transformed cellsC. recombinant cellsD. None of these
• While isolating DNA from bacteria, which of the following enzymes is not used ?A. LysozymeB. RibonucleaseC. DeoxyribonucleaseD. Protease.
• Rising of dough is due isA. multiplication of yeastB. production of `CO_(2)`C. emulsificationD. hydrolysis of wheat flour starch into sugars .
• An enzyme catalysing the removal of nucleotides from the ends of DNA isA. endonucleaseB. exonucleaseC. DNA ligaseD. Hind II