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Competent cells can be prepared using ________(a) electrophoresis(b) chromatography(c) heat-shock treatment(d) pipettingThis question was addressed to me in semester exam.This question is from Tools of Recombinant DNA Technology: Competent Host topic in division Biotechnology Principles and Processes of Biology – Class 12

Answer»

The correct choice is (C) heat-shock treatment

Easy explanation: Heat-shock treatment is a method in which the vial is INCUBATED in ice, then treated at temperature up to 50°C and again TRANSFERRED on ice. It is usually used in transferring recombinant DNA within the HOST (bacterial cell).



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