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Explain Hplc Instrumentation? |
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Answer» It involves solvent system, pump, Sample INJECTOR, HPLC columns, Detectors and Recorder. Firstly, solvent(mobile phase) is DEGASSED for ELIMINATING the bubbles. It is passed through the pump with a uniform PRESSURE. The liquid sample is injected into the mobile phase flow stream. It PASSES through the stationary phase identified by the detectors and recorded. It involves solvent system, pump, Sample injector, HPLC columns, Detectors and Recorder. Firstly, solvent(mobile phase) is degassed for eliminating the bubbles. It is passed through the pump with a uniform pressure. The liquid sample is injected into the mobile phase flow stream. It passes through the stationary phase identified by the detectors and recorded. |
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