Fragments of DNA formed after treatment with endonucleases are separated by the technique of _______(a) polymerase chain reaction(b) gene amplification(c) blotting(d) gel electrophoresisThis question was posed to me in quiz.My enquiry is from Tools of Recombinant DNA Technology: Restriction Enzymes topic in chapter Biotechnology Principles and Processes of Biology – Class 12

Fragments of DNA formed after treatment with endonucleases are separat

1.	Fragments of DNA formed after treatment with endonucleases are separated by the technique of _______(a) polymerase chain reaction(b) gene amplification(c) blotting(d) gel electrophoresisThis question was posed to me in quiz.My enquiry is from Tools of Recombinant DNA Technology: Restriction Enzymes topic in chapter Biotechnology Principles and Processes of Biology – Class 12
Answer» The CORRECT option is (d) gel ELECTROPHORESIS To explain I would say: DNA is a charged molecule. When FRAGMENTED, it can be separated by the technique called gel electrophoresis. It is a technique in which the MOLECULES are separated BASED on the charge present on that molecule.

Discussion

No Comment Found

Related InterviewSolutions

Bioreactors can contain volume up to ______ litres.(a) 20(b) 100-1000(c) 1000000(d) 50This question was posed to me during an interview.Asked question is from Biotechnology topic in section Biotechnology Principles and Processes of Biology – Class 12
The transformants containing ______ gene will grow on plates containing antibiotics.(a) antibiotic-resistance(b) amino acid(c) selectable marker(d) vitamin-resistanceThis question was addressed to me during a job interview.Asked question is from Biotechnology in chapter Biotechnology Principles and Processes of Biology – Class 12
Taq polymerase is obtained from _______(a) S.typhi(b) E.coli(c) Thermus aquaticus(d) ThermophilusI had been asked this question during a job interview.This interesting question is from Biotechnology topic in portion Biotechnology Principles and Processes of Biology – Class 12
What does PCR stand for?(a) Polymerase chain radioactivity(b) Polymerase chitin reaction(c) Phenol carbohydrate reaction(d) Polymerase chain reactionThis question was addressed to me by my college director while I was bunking the class.My doubt is from Biotechnology in division Biotechnology Principles and Processes of Biology – Class 12
The three steps involved in PCR are ________(a) annealing, extension, and denaturation(b) extension, denaturation, and annealing(c) denaturation, annealing, and extension(d) denaturation, extension, and annealingThe question was asked in examination.Question is from Biotechnology in section Biotechnology Principles and Processes of Biology – Class 12
DNA moves towards ______ during agarose gel electrophoresis.(a) negative terminal(b) cathode(c) anode(d) does not moveThis question was posed to me in an international level competition.Question is taken from Biotechnology in section Biotechnology Principles and Processes of Biology – Class 12
Which enzyme helps in joining of DNA?(a) Lyase(b) Ligase(c) Helicase(d) LipaseI have been asked this question by my college professor while I was bunking the class.My question comes from Biotechnology topic in chapter Biotechnology Principles and Processes of Biology – Class 12
Which chemical is used to obtain a precipitate of pure DNA?(a) Methanol(b) Ether(c) HCl(d) EthanolI got this question in an international level competition.My question comes from Biotechnology in chapter Biotechnology Principles and Processes of Biology – Class 12
DNA is _____ charged.(a) positively(b) neutral(c) negatively(d) polarI had been asked this question during an internship interview.This key question is from Biotechnology topic in portion Biotechnology Principles and Processes of Biology – Class 12
The enzyme which cleaves proteins is ______(a) protease(b) RNase(c) lipase(d) DnaseI got this question by my school teacher while I was bunking the class.Question is from Biotechnology topic in portion Biotechnology Principles and Processes of Biology – Class 12

Discussion

No Comment Found

Related InterviewSolutions

Reply to Comment