In case of genome sequence assembly which of the given statement is incorrect?(a) Full chromosomal sequences areassembled from the overlaps in a highly redundant set of fragments by an automatic computational method or from the fragment order on a physical map(b) Chromosome cloning is carried out inbacterial artifical chromosomes (BACs)(c) Chromosomes of a target organism are purified, fragmented, and subcloned in fragments of size hundreds of bp(d) Genome sequences are assembled from DNA sequence fragments of approximate length 500 bp obtained using DNA sequencing machinesI have been asked this question in an internship interview.This is a very interesting question from Sequence Assembly and Gene Identification topic in chapter Collecting & Storing Sequences in Laboratory of Bioinformatics

In case of genome sequence assembly which of the given statement is in

1.	In case of genome sequence assembly which of the given statement is incorrect?(a) Full chromosomal sequences areassembled from the overlaps in a highly redundant set of fragments by an automatic computational method or from the fragment order on a physical map(b) Chromosome cloning is carried out inbacterial artifical chromosomes (BACs)(c) Chromosomes of a target organism are purified, fragmented, and subcloned in fragments of size hundreds of bp(d) Genome sequences are assembled from DNA sequence fragments of approximate length 500 bp obtained using DNA sequencing machinesI have been asked this question in an internship interview.This is a very interesting question from Sequence Assembly and Gene Identification topic in chapter Collecting & Storing Sequences in Laboratory of Bioinformatics
Answer» The correct CHOICE is (C) Chromosomes of a TARGET organism are purified, fragmented, and subcloned in fragments of size hundreds of bp Easy explanation: Chromosomes of a target organism are purified, fragmented, and subcloned in fragments of size hundreds of KBP and not bp.The BAC fragments are then further subcloned as smaller fragments into plasmid vectors for DNA sequencing.

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