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In Protein 2D gel Electrophoresis, Individual proteins produced by the genome can be separated to _____ by this method and specific ones identified by various _______(a) smaller extent, biochemical and immunological tests(b) a large extent, biochemical and immunological tests(c) a large extent, biochemical tests only(d) smaller extent, purely mechanical testsI have been asked this question in an interview.I'm obligated to ask this question of Sequence Assembly and Gene Identification topic in section Collecting & Storing Sequences in Laboratory of Bioinformatics

Answer»

Correct choice is (b) a LARGE EXTENT, biochemical and immunological tests

Easy explanation: Moreover, changes in levels of proteins in response to an environment signal can be monitored in much the same way as a microarray analysis is performed. Microarrays only DETECT untranslated mRNAs, whereas a two-dimensional GEL protein analysis detects translation products, THUS revealing an additional level of regulation.



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